摘要
目的研究自制SMO(上海多器官)液对草犬肝脏低温保存的效果。方法采用草犬肝脏非循环离体灌注模型,比较SMO液、UW(威斯康星大学)液和LR(乳酸林格)液对草犬肝脏保存0、12、24、36、48h后,其形态学及生物化学的改变。并进行肝脏移植,观察移植后移植动物存活时间。结果SMO液保存48h之内,肝脏的形态学观测无明显改变。保存48h的肝脏,其各项生化功能SMO组明显优于LR液组(P<0.05);与UW液组比较无显著性差异(P>0.05),但SLCM(肝窦内皮细胞病死率)、HTWC(肝细胞含水量)值两组间有显著性差异(P<0.05)。保存36、48h后保存液pH值,SMO组明显高于UW组(P<0.05)。SMO液低温保存24h后行原位肝移植5例,5例均活过术后24h。结论SMO液保存犬肝48h效果明显优于LR液,与UW液相当,在改善低温保存及再灌注肝脏能量代谢、预防细胞内酸中毒及肝脏原位灌洗等方面,SMO液略优遇UW液。
Objective To study the effect of self-designed SMO solution on cold prolonged storage of dog lives.Methods The preservation effect of SMO solution with that of UW solution and Lactate Ringers solution （LR） was compared by using isolated perfused dog liver model Results All the indices of hepatic biochemical functions in SMO group were better than that of LR group （P〈0.05）, but no significant difference with that of UW group for 48h storage （P〉0.05）. pH in SMO group was markedly higher than in UW group for 36 or 48h storage （P〈0.05）. Conclusion The effect on 48h storage of dog livers with SMO solution might be better than with LR solution. The effect of SMO solution is similar to that of UW solution within 48h storage. However, SMO solution is slightly superior to UW solution in some aspects, such as improvement of energy metabolism during cold storage and reperfusion, prevention of intracellular acidosis and perfusion of liver in situ.
出处
《实用医药杂志》
2007年第4期465-467,共3页
Practical Journal of Medicine & Pharmacy
关键词
肝脏
器官保存
移植
Liver Organ preservation Transplantation
