摘要
从油菜植株体内分离出的内生细菌BY-2,经过生物学鉴定为枯草芽孢杆菌(Bacillus subtilis)。用BY-2回接油菜,重新分离到具有BY-2相同形态特征和抑制病原真菌能力的内生菌株。油菜接种后的第10d,体内的BY-2菌数达(2.24～9.02)×103cfu/g鲜植株,25d仍然保持在(3.13～8.59)×103cfu/g鲜植株。BY-2与油菜核盘菌[Sclerotinia sclerotiorum(Lib.)de Bary]对峙培养可以形成直径为3.1cm的抑菌圈;可使油菜核盘菌菌丝细胞浓缩变短,细胞壁破裂,原生质外溢,从而抑制真菌生长发育;同时还能抑制菌核的萌发,抑制率达60%～70%;在油菜离体叶片试验中,BY-2对菌核病的防治效果达100%。
Endophytic Bacillus subtilis BY-2 was isolated from oilseed rape. After return inoculation treatment to oilseed rape, the same endophytic B. subtilis as BY-2 was obtained. In 10 d after inoculation, the number of endophytic bacterium reached up to （2.24 - 9.02 ）× 10^3 cfu/g fresh plant. 25 d later, the number of bacterium was maintained （3.13 -8.59）×10^3 cfu/g fresh plant. The control effect of BY-2 on Sclerotinia sclerotiorum was investigated. After 2-day-confronting incubation on PDA plates, the inhibition zone reached to 3.1 cm. Meantime, serious mycelium malformation of S. sclerotiorum caused by BY-2 was observed, such as short growth, cytoplasm condensation, cell wail break and protoplasm leak. Even germination of sclerotia was also inhibited, the inhibition rate reached to 60% -70%. The control efficacy of BY-2 against S. sclerotiorum was 100% on detached leaves in vitro.
出处
《植物病理学报》
CAS
CSCD
北大核心
2007年第2期192-196,共5页
Acta Phytopathologica Sinica
基金
国家"十五"科技攻关项目(2004BA520A16-03)
湖北省科技攻关项目(2002AA201C02)
关键词
油菜
内生细菌
油菜体内定殖
菌核病菌
对峙培养
oilseed rape
endophyte bacterium
colonization trend
Sclerotinia sclerotiorum
confronting incubation
