摘要
目的观察静脉注射骨髓基质干细胞（MSCs）对放射性脑损伤模型大鼠认知功能的影响。方法离体分离、培养、增殖成年大鼠MSCs。采用6 MV X线对8～10周龄雌性SD大鼠做全脑照射，照射剂量20 Gy。照后1周采用尾静脉注射Hoechst33342荧光标记的MSCs（4×10^6），注射后4、8周观察大鼠认知功能变化，应用免疫荧光技术检测大鼠脑内Hoechst33342标记细胞及分化的功能细胞。结果与正常对照组相比，模型组大鼠的学习记忆能力明显受损（P＜0．01），静脉注射后4、8周，照射组大鼠认知功能明显改善（P＜0．05）。结论静脉注射MSCs可显著改善全脑照射大鼠认知功能，MSCs可在放射性脑损伤大鼠脑组织中存活和分化。
Objective To explore the effect of intravenous infusion of hone marrow stromal stem cells（MSCs） on cognitive function of rats after whole brain irradiation. Methods MSCs were isolated and cultured from adult rats. After Sprague-Dawly female rats were anaesthetized with chloral hydrate, their whole cerebrum was irradiated with a single dose of 20 Gy by 6 MV X-ray. Seven days after irradiation, 4×10^6 Hoechst33342-labelled MSCs were intravenously injected into the tail vein of these rats. Four and 8 weeks after transplantation, the learning and memorizing ability was measured with the Y maze test. Immunohistochemical method was used to identify MSCs or cells derived from MSCs in the brain. Results The learning and memorizing ability of irradiation groups were significantly different from that of normal control group （P 〈 0. 01）. Significant improvement of cognitive impairment was observed in rats treated with MSCs at 4 and 8 weeks after transplantation as compared with the controll groups （P 〈 0.05）. This showed that the MSCs survived and were localized to the brain tissue. The number of Hoechst33342 immunohistofluorescence positive cells and double-immunostaining cells significantly decreased in 8 weeks group as compared with the 4 weeks group. Conclusion Marrow stromal stem cells delivered to the irradiation brain tissue through intravenous route improve the cognitive impairment after whole brain irradiation. These cells may survive and differentiate in the brain tissue of irradiated rats.
出处
《中华放射肿瘤学杂志》
CSCD
北大核心
2007年第2期143-146,共4页
Chinese Journal of Radiation Oncology
基金
浙江省卫生厅资助项目（2005A109）
关键词
骨髓基质干细胞
细胞移植
照射
认知
大鼠
Bone marrow stromal stem cells
Cell transplantion
Irradiation
Cognition
Rat
