摘要
目的观察单次全脑照射大鼠大脑皮质水通道蛋白4（AQP4）表达的变化，探讨AQP4在早期放射性脑损伤中的作用。方法将雄性SD大鼠100只随机分为2、10、30 Gy单次全脑照射后1d、1周和1个月及对照10个组别，构建放射性脑损伤大鼠脑组织芯片，用免疫组化法检测皮质AQP4蛋白表达变化。结果与对照组比较，2 Gy照射后各时间点皮质AQP4表达水平无明显变化；10、30 Gy照射后1 d皮质AQP4表达迅速降低，照后1周达最低点（P＜0．01），1个月时表达水平又明显高于对照组（P＜0．05）；随着受照剂量增加AQP4表达也明显降低，30 Gy组达最低点（P＜0．01）。结论大鼠全脑照射后脑皮质AQP4表达呈时程性和剂量依赖性变化；早期放射性脑损伤中AQP4表达下降是一种保护性反应，具有减轻脑水肿效应。
Objective To investigate the early changes of aquaporin-4（AQP4） expression in the cortex of rats following whole brain irradiation and its role in the early phase irradiation-induced brain injury. Methods 100 male Sprague-Dawley rats were randomly divided into sham-irradiation group, and irradiation group. One day, 1 week and 1 month after whole brain irradiation of these rats with a single-dose of 2, 10 and 30 Gy by 4 MeV electron beam. Tissue microarray of radiation-induced brain injury in rats was constructed and AQP4 expression in the cortex was examined by immunohisto-chemical method. Results Compared with the sham-irradiation group, AQP4 expression in the cortex of rats in the 2 Gy group showed no obvious change. However, AQP4 expression in 10 Gy and 30 Gy groups decreased quickly in D1 post-radiation and reached the lowest point one week post-radiation. Then, it increased slowly and the level of AQP4 expression in M1 was obviously higher than those of the sham-irradiation group （P 〈 0.05）. AQP4 expression lowered in a manner of dose-dependent after whole brain irradiation and reached the lowest point at 30 Gy. Conclusions Aquaporin-4 expression in the rat cortex changed at in a time-and-dose-dependent manner after whole brain irradiation. After irradiation, the lowered AQP4 expression is a protective response to blood-brain barrier, as it could reduce cerebral edema.
出处
《中华放射肿瘤学杂志》
CSCD
北大核心
2007年第2期140-142,共3页
Chinese Journal of Radiation Oncology
基金
江苏省卫生厅重大科研课题（K200406）
