摘要
[目的]建立高效液相色谱法测定癌痛胶囊中人参皂苷Rb1的含量方法。[方法]采用HP1100高效液相色谱仪(包括G1314A可变波长紫外检测器,HP化学工作站,G1322A真空脱气机,HP1100四元泵),Hypersil ODS柱(4.6mm×250mm,5μm)。流动相:乙腈-水(30∶70),紫外检测波长:203nm,流速:1.0ml/min,进样量20μl。[结果]人参皂苷Rb1在0.04μg^2μg范围内呈现良好的线性关系,回收率为98.26%(n=5),RSD为1.41%。[结论]该法简便、准确、具有专属性,可用于测定癌痛胶囊中人参皂苷Rb1的含量。
[Objective] To establish n HPLC method for analysis of ginsenoside Rb1 in AITONG capsules. [Method]The HPLC system consists of G1314A variable wavelength un-visible detector, Hypersil ODS（4. 6min×250mm, 5μm）, HP chemstation, Hp1100 Series Quaternary Pump and Vacuum degasser （G1322A）. The mobile phase was： Acetonitrile：Water （30 ： 70,v/v）. The flowrate was 1.0ml·min^-1 ,the injection volume was 20/,1,and the detector wavelength was 203nm. [Results] The method was linear at the ginsenoside Rb1 ranging from 0. 04μg to 2μg. The recovery of method was 98. 26%（n=5） ,the average RSD was 1.41% .
出处
《浙江中医药大学学报》
CAS
2007年第2期209-210,共2页
Journal of Zhejiang Chinese Medical University
