摘要
本实验采用肿瘤坏死因子（ＴＮＦ）细胞毒生物测定法及巨噬细胞酸性磷酸酶（ＡＣＰ）生化检测法，观察了经单一因素和复合因素预处理的小鼠在接受致死量内毒素（ＬＰＳ）注射后血清ＴＮＦ水平及腹腔巨噬细胞ＡＣＰ含量的变化。结果表明。当腹腔注射ＬＰＳ９０分钟后，各组小鼠血清ＴＮＦ均有不同程度的升高，生理盐水组最明显（Ｐ＜０．０１）。氢化考的松对ＬＰＳ体内诱生ＴＮＦ具有明显的抑制效应（Ｐ＜０．０１），其巨噬细胞ＡＣＰ值与血清ＴＮＦ水平具有明显的直线相关关系（Ｐ＜０．００２５）。结果提示：小鼠腹腔巨噬细胞活性与其分泌ＴＮＦ强度间可能具有一定的联系。承气合剂组血清ＴＮＦ值低于生理盐水组；但其巨噬细胞ＡＣＰ却明显高于其它各组。本文通过分析血清ＴＮＦ值与巨噬细胞活性间的这种不协调性，阐述了承气合剂所具有的直接或间接地抑制巨噬细胞释放过程的可能性。
To study the action of Tumor Necrosis Factor(TNF)secretion of murine peritoneal macrophages challenged by LPS which was combined or noncombined with other drugs in vivo and to analyse the relationship between macrophage activity and TNF secretion, the methods of TNF cytotoxity bioassay and ACP biochemical measurement were used to investigate the changes of serum TNF and peritoneal macrophage ACP after pretreament with the drugs.The results showed a varying extent of elevation of serum T NF at 90 minutes after intraperitoneal injection of lethal dose of LPS,especially in the normal saline group (P<0.001).Hydrocortisone manifested an obvious inhibitive effect on LPS-induced serum TNF(P<0.001),The findings indicated that there might be some correlation between the activity of macrophages and TNF serection.It was also demonstrated that the content of serum TNF in Cheng Qi group was lower than that in normal saline group (P<0.01),but in the quanitity of ACP was obviously higher than other groups. Through discussing the incoordination the possibility was expoundetl that there might be an inhibition to the release process of murine peritoneal macrophages in Cheng Qi group directly or indirectly.
出处
《中国中西医结合外科杂志》
CAS
1996年第1期39-44,共6页
Chinese Journal of Surgery of Integrated Traditional and Western Medicine
