摘要
应用水热法合成质谱专用提取血清多肽的金属螯合纳米磁珠,可用于质谱对血清中多肽分布情况的研究,获得血清多肽谱．对磁珠进行透射电镜(TEM),原子力显微镜(AFM)和傅立叶红外光谱FT-IR的表征,显示该粒子粒径在70～90 nm．并通过质谱验证该金属螯合磁珠能有效提取血清中的多肽,该磁珠为质谱进行疾病诊断解决样品制备的技术难题,具有广阔应用前景．
A novel hydrothermal route has been developed to produce metal-chelated magnetic nanobeads which help to study serum peptides profiles by matrix-assisted laser desorption/ionization time-of-flight （MALDI-TOF） mass spectrum. The obtained particles were analyzed by TEM, AFM and FT-IR techniques, and it showed that the average particle size ranged between 70-90 nm. It is validated by mass spectrum that the metal-chelated magnetic nanobeads can extract serum peptides in an effective way. These beads are very useful in the clinic studies on serum preparation for mass spectrum in future.
出处
《化学学报》
SCIE
CAS
CSCD
北大核心
2007年第4期344-348,共5页
Acta Chimica Sinica
基金
国家973计划(No.2006cb500700)资助项目.
关键词
热水解法
质谱
多肽
金属螯合磁珠
hydrothermal method
mass spectrum
peptide
metal-chelated magnetic nanobead
