摘要
基于磁珠分离的生物质谱技术,建立急性白血病疗效的血清评价方法。通过对质控血清的连续检测分析,日间及日内重复性均达国际标准,确保血清多肽分析体系的稳定性及可靠性。以30例初发急性白血病血清及其治疗缓解血清为研究对象,经金属铜鳌合磁珠分离和基质辅助激光解吸电离飞行时间质谱仪(MAL-DI-TOF-MS)检测后获得血清多肽谱图。经过FlexAnalysis 2.4软件和Biosun_MS软件分析,在m/z1000～10000范围内,得到27个具有统计学意义(p<0.05)的差异峰,其中治疗缓解后表达上调峰10个,表达下调峰17个,利用上述差异峰建立的诊断模型获得了100%敏感性和90%的特异性。基于磁珠分离和MALDI-TOF-MS检测建立的诊断模型具有较高的敏感性和特异性,对差异峰进行测序鉴定将有助于急性白血病疗效评价和发病机理研究。
A serum method for evaluating therapeutic efficacy of acute leukemia has been built by magnetic beads-based biological mass spectrometric technology.After analysis of quality control sample,the reproduci-bility of within-run assay and between-run assay was obtained and consistent with the previous reports.This result manifested that the method used for serum peptides analysis was stable and reliable.Serum samples from 30 patients with primary acute leukemia and those with complete remission after treatment were extracted by metal-chelated magnetic beads and detected by MALDI-TOF-MS.Peaks at the m/z range 1000-10000 were analyzed by FlexAnalysis 2.4 software and Biosun_MS software.Approximately 27 differential peaks that were of statistical significance（p0.05） were obtained.Ten peaks were up-regulated and 17 peaks were down-regulated after complete remission,respectively.The diagnostic model based on these differential peaks achieved 100% sensitivity and 90% specificity.The results indicated that the model based on magnetic beads extraction and MALDI-TOF-MS detection acquires high sensitivity and specificity.Further sequence identification of differential peaks is useful for evaluation of therapeutic efficacy and mechanism research on acute leukemia.
出处
《分析化学》
SCIE
EI
CAS
CSCD
北大核心
2010年第2期233-236,共4页
Chinese Journal of Analytical Chemistry
基金
吉林省科技厅白求恩专项基金项目(No.200705103)
2007-2009年度卫生部临床重点学科项目(常见恶性肿瘤分子标志的临床评价--血清多肽图作为恶性肿瘤早期标志的临床评价)
创新工作方法资助项目(No.2008IM022000)
支撑计划项目(No.2008BAK14B04)
新药创制项目(No.2009ZX09301-002)资助
关键词
磁珠
质谱
急性白血病
治疗
Magnetic beads Mass spectrometry Acute leukemia Treatment
