摘要
目的:探讨多肿瘤标志物蛋白芯片技术对卵巢癌诊断的应用价值。方法:应用多肿瘤标志物蛋白芯片技术测定66例卵巢癌患者,64例妇科良性病患者和144例健康女性的血清12项肿瘤标志物(CA19-9、NSE、CEA、CA242、CA125、CA153、AFP、Ferritin、f-PSA、PSA、β-HCG、HGH)的水平,并评价蛋白芯片的诊断价值。结果:卵巢癌组的血清CA19-9、CEA、CA242、Ferritin、AFP、CA125和CA153水平显著高于妇科良性病组和健康对照组(P<0.01)。卵巢癌组CA125、Ferritin、CA19-9、CEA、CA242和CA153单项指标的阳性率依次分别为68.2%、31.8%、30.3%、22.7%、19.7%和19.7%,与妇科良性病组和健康组比较有显著性差异(P<0.001)。蛋白芯片12项指标联合检测的阳性率显著高于任何单项标志物(P<0.001)。蛋白芯片检测中CA125+CA19-9二项和CA125+CA19-9+CEA三项联合检测的诊断敏感性和准确性明显高于其CA125单项指标。蛋白芯片12项指标联合检测的诊断敏感性、特异性、准确性、阳性预测值和阴性预测值分别为87.9%、93.3%、92.0%、80.6%和96.0%。与其CA125单项指标比较,诊断敏感性和准确性分别从68.2%和90.5%增加到87.9%和92.0%。结论:应用蛋白芯片技术联合检测肿瘤标志物有效提高诊断敏感性和准确性,对卵巢癌的辅助诊断具有重要临床应用价值。
Objective：To investigate the application of multi-tumor marker protein chip in the diagnosis of ovarian cancer. Methods：The serum levels of 12 tumor markers including CA125,CA19-9,CA153 ,CA242,CEA, AFP, β-HCG, NSE, PSA, f-PSA, ferritin and HGH were determined by using multi-tumor marker protein chip in 66 patients with malignant ovarian cancer,64 patients with benign gynecologic diseases and 144 healthy women,and its diagnostic value was evaluated. Results：The levels of CA19-9, CEA, CA242, ferritin, AFP, CA125 and CA153 in patients with ovarian cancer were significantly higher than those in patients with benign diseases and healthy controls （P 〈 0. 01 ）. The positive rates of CA125, ferritin, CA19-9, CEA, CA242, CA153 were 68.2%, 31.8%, 30. 3%, 22. 7%, 19. 7% and 19. 7% in patients with ovarian cancer, respectively, which were significantly higher than those in benign diseases and healthy controls （P 〈0. 001 ）. The protein chip had significantly higher positive rate than any single item of the 12 tumor markers （ P 〈 0. 001 ）. The diagnostic sensitivity and accuracy of the combined detection of CA125 ＋ CA19-9 and CA125 ＋ CA19-9 ＋ CEA were much higher than those of single CA125 for ovarian cancer. The sensitivity, specificity, accuracy, positive and negative predictive values by protein chip detection were 87.9%, 93.3%, 92. 0%, 80. 6% and 96. 0%, respectively. As compared with CA125 alone, the diagnostic sensitivity and accuracy of the protein chip increased from 68.2% and 90. 5% to 87.9% and 92. 0%, respectively. Conclusion ：The combined detection of tumor markers by protein chip technique is helpful in improving the diagnostic sensitivity and accuracy and valuable for the diagnosis of ovarian cancer.
出处
《临床肿瘤学杂志》
CAS
2007年第3期194-197,共4页
Chinese Clinical Oncology
