摘要
目的探寻卵巢癌诊断的理想肿瘤标志物,以提高卵巢癌筛查的准确性和灵敏度。方法荧光定量PCR(FQ-PCR)检测测定20例卵巢良性变和85例卵巢癌患者基因突变型p53拷贝数,ELISA法检测血清中肿瘤相关抗原(CA125)、癌胚抗原(CEA)、恶性肿瘤特异性生长因子(TGSF)。结果 CA125、CEA、肿瘤特异性生长因子(TSGF)和p53,4种标志物的水平及阳性率明显高于良性组(P<0.05),在几种组合中,CA125、TGSF、p53三者组合在Ⅰ、Ⅱ期卵巢癌中特异性达54.1%,灵敏度达88.5%。结论 CA125、TGSF和p53联合是卵巢癌筛查较好的组合。
Objective To explore the best tumor marker combination in diagnosis of early stage ovary cancer and to get higher accuracy and sensitivity.Methods Serum levels of CA125,CEA and TGSF were detected by enzyme linked immunosorbent assay in 20 cases of benign ovary diseases and 85 cases of ovary cancer.Meanwhile mutant genotype p^53 was detected by FQ-PCR.Results The serum levels and positive rates of CA_125,CEA,TGSF and mutant p53 in patients with ovary cancers were significantly higher than those of patients with benign ovary tumor（P〈0.05）.The combined detection of CA125,TGSF and mutant genotype p53 had higher positive rate（90.8%） than others in patients with I,Ⅱ stage ovary cancer.Conclusion The combined detection of CA125,TGSF and p^53 is an effective method for screening ovary cancer.
出处
《临床和实验医学杂志》
2012年第5期341-343,共3页
Journal of Clinical and Experimental Medicine
