摘要
在Ham’s F-10培养液中,添加了不同剂量的己酮可可碱(PF)或血小板激活因子(PAF),与大熊猫冷冻保存后解冻的精子在37℃水浴中孵育,通过测量孵育不同时间精子的活力、存活时间、顶体反应率、质膜完整率和异种穿卵率,来探讨PF、PAF对大熊猫冷冻精子体外受精能力的影响,结果显示:己酮可可碱、血小板激活因子均能影响大熊猫冷冻精子体外受精能力。其中:己酮可可碱以1 mg/mL处理剂量对提高大熊猫冷冻精子体外受精能力效果较好,总存活时间达(15.33±4.73)h,培养4h时体外异种穿卵率达51.44%,培养6 h时体外异种穿卵率为7.49%,与对照组差异极显著(P<0.01),并优于其它处理组;PAF 50 ng/mL处理剂量下的各质量评估指标优于100 ng/mL处理,但用PAF处理精液的时间超过2 h后即引起精子质量的显著下降。
Post-thaw Giant Panda sperm was incubated in Ham＇s F-10 medium with different dosage pentoxifylline （PF） or platelet activated factor （PAF） under 37 ℃. The effects of PF or PAF on giant panda post-thawed sperm fertilizing capability were evaluated by spermatozoa motility, survival time, acrosome reaction ratio, membrane integrity ratio and heterogenetic penetrationegg ratio. The results showed： PF and PAF affected giant panda post-thaw sperm fertility in vitro. In the experiment, 1 mg/mL PF was best for improving giant panda post-thaw sperm in vitro fertilizing capability. When giant panda post-thaw sperm was treated with lmg/mL PF under 37 ℃, the survival time of sperm was （15.33±4.73）h, the heterogenetic penetration-egg ratio was 51.44% after incubating for 4 hours, the heterogenetic penetration-egg ratio was still 7.49% after incubating for 6 hours. The results were significant higher than control group（P〈 0.01）, and better than other treated groups; When giant panda post-thaw sperm was treated with PAF, sperm quality in 50 ng/mL group was better than that in 100 ng/mL group, but the sperm quality would obviously decrease when incubation time exceeded 2hours.
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
2007年第3期294-300,共7页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金
中国大熊猫繁育研究基金(2003010)
关键词
大熊猫
冷冻精液
受精能力
己酮可可碱
血小板激活因子
giant panda（Ailuropoda melanoleuca ）
frozen-sperm
fertilizing capacity： pentoxilylllne （PF）
platelel activating factor （PAF）
