摘要
以纯化的ＨＳＰ２７和ＨＳＰ７０通过ＷｅｓｔｅｒｎＢｌｏｔ检测接触不同浓度苯和苯中毒工人血浆中相应抗体水平。结果表明：苯中毒组ＨＳＰ７０抗体水平高于其它任一组，其它组间无显著性差异；而ＨＳＰ２７抗体水平无组间差异，提示ＨＳＰ７０抗体水平均高可能与苯及其代谢产物使细胞应激反应增强有关。Ｐ＜０．０１分别与各组比较。讨论热应激蛋白是原核生物和真核生物产生的具有高度保守性的蛋白家族。细胞在热、创伤、感染、重金属、化学物质、自由基和能量代谢抑制剂等多种因素作用下都被诱导产生热应激蛋白［３］。本实验结果表明，苯中毒组血浆ＨＳＰ７０抗体水平明显高于对照组。苯是明确的致癌剂，其对机体的慢性毒作用主要是通过中间代谢产物作用于造血系统。其重要的中间代谢产物主要有环氧化苯、氢醌和邻苯二酚［６］。我们检测发现苯中毒组的ＨＳＰ７０抗体水平明显高于其它组。ＨＳＰｓ抗体产生机制有两点与苯的毒作用机制一致，即抗原的变异和隐蔽抗原的释放及遗传因素所致［７］。苯及其代谢产物对细胞的直接刺激可使ＨＳＰｓ水平升高。另外，苯在代谢过程中还可产生一些自由基［８］，自由基可诱导ＨＳＰｓ产生。有报道，暴露于苯的大鼠肝、骨髓及红细胞ＳＯＤ活性高于对照组（Ｐ＜?
Antibodies to HSP27 and HSP70 in plasma of benzene exposed workers were detected using cor responding purified HSPs with Western Blot. 100 workers were divided into benzene poisoned group, high level group, low level group and control group. It was showed that the positive occur rence rate of antibody to HSP70 of poisoned group was higher than those of other groups, However, no differenee existed among any other groups,These results suggested that high level antibody to HSP70 may be associated with benzene stress to white blood cells.
出处
《工业卫生与职业病》
CAS
CSCD
1996年第2期83-85,共3页
Industrial Health and Occupational Diseases
基金
国家自然科学基金
关键词
苯
热应激蛋白抗体
Benzene
Antibodies to HSPs
Western Blot
