摘要
本文记述了微孢子虫3新种。槐尺蠖微粒子虫Nosema semiothisae sp. nov.,自然寄主:槐尺蠖Semiothisa cinerearia Bremer & Grey,实验寄主:9种鳞翅目昆虫;银纹夜蛾微粒子虫N. agnatae sp. nov., 自然寄主:银纹夜蛾Argyrogramma agnata(Staudinger),实验寄主:15种鳞翅目昆虫;银纹夜蛾八孢虫Microsporidium argyrogrammi sp. nov., 自然寄主:银纹夜蛾。除描述了它们光镜与电镜形态特征之外,并与近似种比较,作了简短讨论。
Three new species of microsporidia from two lepidopterous insects are described in this paper. The type slides are deposited in the Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing.Nosema temiothisae sp. nov.(pl. I, figs. 1-5)Host and Site: (Natural) Semiothisa cinercaria (B. et G.), General infection in all stages; (Experimental) Heliothis armigcra H., H. assulta G., larvae, infection in many tissues, most in midgut, Stilpnotia Candida Spodoptera litura F.,Bombyx mori L., Pieris rapae L., Agrotis ipsilon (H.) and Ostrinia furnocalis (G.); but Lymantria dispar L., Mythimna separata (W.) and Clostera enachoreta (F.) were refractory to the infection.Lesion Infected tissues appear white and hypertrophied, the foci of silk glands of the larvae of O. furnacalis appear cloudy or misty-white, with blurry boundary (fig. 5).Life Cycle Monomorphic. Schizonts consist of 2, 4 or 8 diplokarya forms, sporonts rhombic with 2 diplokarya. A sporont undergoes binary fission to produce 2 sporoblasts that transform into 2 spores (figs, 1-2).Spore When fresh, most were elongate pyriform with a mean size of 3.94±0.19×2.02± 0.16 μm (n = 50). The polar filament was normally arranged in 10-11 coils at angles of 70-80?to the axes of spores, with a mean length of :76.3±3.9 μm (n = 16) (fig. 3).Discussion N. semiothisae is distinct from other Nosema spp. of Lepidoptera by having elongate pyriform spores with the exception of N. wistmansi which also has elongate pyriform spores, but can be distinguished from the latter by having smaller spore size. In addition, N. semiothisae can infect the silk glands while the latter can not.Type locality Dongberwang, 39°9'N,116°4'E Beijing Sept. 1984Nosema aynatae sp. nov. (pl. Ⅱ, figs. 6-16)Host and Site (Natural) Argyrogramma agnata S., General infection in allstages; (Experimental) 0. furnacalis (G.), infection in all stages, many tissues except fat body; P. rapac L., Euproctis pseudoconspersa S., Cnaphalocrocis medinalis G., H, armigera H., H. assuha (G.), A. ipsilon (H.) M. separata (W.), S. Candida S., C. anachoreta (F.), S. litura F., B. mori L. L. dispar L. and Philosemia cythia ricinia D.Lesion The foci in silk glands and salivary glands appear snowywhite and hypertrophied with clear boundary (fig. 16). Infected fatbody in Euprociis pseudoconspersa was destroyed into granuliform.Life Cycle Monomorphic. Schizonts typically round with 2, 4 or 8 nuclei in diplokaryon arrangement, octonucleate lopsided forms possibly of pre-sporont stage, A sporont with two diplokarya gives rise to two sporoblasts (figs. 6-13).Spore Fresh spores ellipsoidal, with a minor tip at an end, 4.06±0.10×1.92 ± 0.15 μm (n = 50) (fig. 14). Polar filarment normally arranged in 13 coils, at angles of 70± to the axes of spores with a mean length of 67.1°3.0μm (n = 30) (fig. 15).Discussion In comparison of host range, tissue specificity and spore size, the new species is similar to other 5 Nosema spp. from Lepidoptera, namely, N. invadens, N. pereziodes, N. disstriae, N. fumiferanae and N. manierae, but can be distinguished from them by having irregular shaped schizonts and distinct ultrastructure in spore. N. invadens and N. pereziades can develop inflammatory responses to infection, N. disstriae and N. fumiferanae mainly infect the midgut, while N. agnatae differs from these habits.Type locality Zhongmou (34°7'N, 114.0°E), Henan Province, Sept. 1984.Microsporidium argyrogrammi sp. nov.(figs. 17-20)Host and Site (Natural) Argyrcgramma agnata (S.), Larvae, fatbody, which was entirely destroyed into granuliform.Life Cycle In 26°C, only monomorphic was seen. Schizont with two unpaired nuclei, sporonts with 2, 4 or 8 unpaired nuclei which produce 8 sporoblasts and transform into 8 uninucleate spores within a persist pansporoblast membrane (figs. 17-19)Spore: Fresh octospores ovoidal, 4.73±0.46×2.95±0.20μm. Polar filarment arranged in 17-18 coils with a uniform diameter throughout (fig. 20). Pansporoblasts spherical, 8-9 μm in diameter.Discussion This species seems to be a member of the
出处
《动物分类学报》
CSCD
1990年第3期257-261,共5页
Acta Zootaxonomica Sinica
基金
国家自然科学基金
关键词
微孢子虫
槐尺蠖
银纹夜蛾
Nosema semiothisae sp. nov. N. agnatae sp. nov. Microsporidium argy- rogramma sp. nov. Semiothisa cinerearia Argyrogramma agnata
