摘要
选择性分离了Ｎｏｓｅｍａｂｏｍｂｙｃｉｓ孢子表面蛋白和总蛋白，采用ＳＤＳ－ＰＡＧＥ检测发现，孢子表面蛋白由分子量不同的３种亚基（３２０００、２６０００、２４５００Ｄａ）组成，其总蛋白至少包括２５个条带。同时将从养蚕生产中收集到的两种微粒子ＭＡ－１和ＭＤ－１以及从野外昆虫收集到的微孢子虫Ｐｈａ－Ｍ和Ｈａ－Ｍ与Ｎ．ｂｏｍｂｙｃｉｓ进行了比较，发现不同微孢子虫间蛋白组成有差异。
Exosporal and total proteins of the spores of Nosema bombycis were selectively separated and analysed with SDS-PAGE.The exosporal protein of N.bombycis consists of three subunits with the molecular weight of about 32 000,26 000 and 24 500 Daltons respectively.The SDS-PAGE profiles of total proteins of the N.bombycis spores contain at least 25 bands,At the same time,microsporidias MA-1 and MD-1,collected from commercial silkworm rearing,Pha-M and Ha-M,from mulberry insect pests Phyllobrotica armata and Hemerophila atrilineata respectively,were compared with N.bombycis on their exosporal and total proteins.Difference in protein components was found among those microsporidias.
出处
《蚕业科学》
CAS
CSCD
1995年第4期238-242,共5页
ACTA SERICOLOGICA SINICA
关键词
家蚕
微孢子虫
孢子表面蛋白
孢子总蛋白
Bombyx mori Microsporidia Exosporal protei Total sporal protein
