摘要
目的:探讨白细胞介素-1β(IL-1β)对精氨酸升压素(AVP)诱导下大鼠心肌成纤维细胞(CFs)诱导型一氧化氮合酶(iNOS)-一氧化氮(NO)系统活性的影响。方法:胰酶消化法分离培养SD仔鼠CFs,硝酸还原酶法、分光光度法和逆转录—聚合酶链式反应(RT-PCR)分别测定不同浓度IL-1β与AVP协同作用下CFs的NO含量、NOS活性和iNOS mRNA表达。结果:AVP诱导下CFs iNOS mRNA表达、NOS活性和NO合成均显著增加(P<0.05)。一定浓度范围内IL-1β与AVP协同作用,剂量依赖性地增加AVP对CFs iNOS-NO系统活性的提高作用,其中AVP+3ng/ml和AVP+5ng/ml IL-1β组的iNOS mRNA表达、NOS活性和NO合成均显著高于AVP组(P<0.05),但IL-Iβ浓度增加至5 ng/ml时,CFs的iNOS mRNA表达、NOS活性和NO合成不再继续升高,反而有所下降。结论:在一定浓度范围内IL-1β可与AVP协同提高CFs iNOS-NO系统活性。
Aim. To explore the effects of interleukin-1β （ IL-1β ） on inducible nitric oxide synthase（ iNOS）-nitric oxide （NO） system activity in arginine vasopressin（AVP）-induced rat cardiac fibroblasts（CFs）. Methods： CFs were isolated by trypsin digestion method. Nitric acid reductase method, spectrophotometry and reverse transcription-polymerase chain reaction （RT-PCR） were used to detect NO contents, NOS activity and iNOS mRNA expression. Results- AVP significantly increased iNOS mRNA expressions, NOS acticity and NO contents（ P 〈 0.05 ） in CFs. IL-1β enhanced the effects of AVP on iNOS-NO system activity in a concentration-dependent manner, moreover the iNOS mRNA expressions, NOS activity and NO contents of AVP＋ 3 ng/ml,AVP＋ 5ng/ml IL-1β group were both significantly higher than those of AVP group（P〈0.05）. But when IL-1β concentration increased to 5ng/ml, the iNOS mRNA expressions, NOS activity andNO contents did not increase accordingly, slightly decreased instead. Conclusion： Within certain range of concentrations IL- 1β cooperates with AVP to increa iNOS-NO system activity in CFs.
出处
《中国应用生理学杂志》
CAS
CSCD
北大核心
2007年第1期70-73,共4页
Chinese Journal of Applied Physiology
