摘要
目的研究一氧化氮合酶(nitricoxidesynthase,NOS)竞争性抑制剂L-硝基精氨酸甲酯(NG-nitro-L-argininemethylester,L-NAME)对失神经肌萎缩的延缓作用,为失神经肌萎缩的预防与治疗提供新手段。方法制备36只成年SD大鼠右下肢腓肠肌失神经支配模型,随机分为L-NAME组(A组)和对照组(B组)。A组:腓肠肌注射L-NAME,于5个不同位点分别注射20μl,总注射剂量为10mg/kg;B组:注射同等体积的生理盐水。于术后2、4和8周测定肌湿重维持率、肌细胞横切面积、肌肉蛋白含量、细胞凋亡数,并观察超微结构的改变。结果术后2、4周A组的肌湿重维持率、肌细胞横切面积、肌肉蛋白含量均明显高于B组,但细胞凋亡数低于B组,差异均有统计学意义(P<0.05)。超微结构显示,术后2周,肌细胞线粒体、内质网及细胞核改变不明显,两组间无显著性差异;术后4周,A组退变的细胞核较B组少,核质染色均匀。术后8周两组各参数间差异无统计学意义(P>0.05)。结论NOS抑制剂L-NAME能在短期内延缓失神经肌萎缩。
Objective To study the effect of the competitive inhibitor of nitric oxide synthase N^G-nitro-L-arginine methyl ester （L-NAME） on the denervated muscle atrophy. Methods A model of the denervated gastrocnemius at the right lower limb was established in 36 SD adult rats. The rats were randomly divided into two groups： the L-NAME group （Group A） and the control group （Group B）. L-NAME 10 mg/kg daily was injected into the denervated gastrocnemius in Group A, and normal saline was injected into the denervated gastrocnemius in Group B. At 2, 4 and 8 weeks after operation, the rate of the muscle wet weight preservation, the cross section area of the myocyte, the protein amount, and the percentage of the apoptotic muscle cells were measured respectively and the ultramicrostructure of the myocyte was observed. Results At 2 and 4 weeks after operation, the rate of the muscle wet weight preservation, the cross section area of the myocyte, and the protein amount were significantly greater in Group A than in Group B; however, the percentage of the apoptotic muscle cells was significantly smaller in Group A than in Group B. The observation of the ultramicrostructure of the myocyte showed that an injection of L-NAME could protect the uhramicrostructure of the myocyte. At 8 weeks after operation, there was no significant difference between the two groups in the above-mentioned parameters. Conclusion The nitric oxide synthase inhibition can delay the denervated muscle atrophy.
出处
《中国修复重建外科杂志》
CAS
CSCD
北大核心
2006年第8期805-808,共4页
Chinese Journal of Reparative and Reconstructive Surgery
关键词
一氧化氮合酶抑制剂
失神经
肌萎缩
Nitric oxide synthase inhibition Denervation Muscle atrophy
