摘要
为获得抗芜菁花叶病毒病的大白菜植株,用携带TuMV-cp基因的农杆菌R1000和EHA105转化大白菜子叶和下胚轴,PCR、PCR-Southern检测证实TuMV-cp基因已导入并整合到大白菜的基因组中.RT-PCR检测表明TuMV-cp在转录水平上获得了稳定表达.TuMV-cp基因在转基因植株T1代中获得了稳定的遗传.抗病性鉴定结果表明转基因植株T1代较非转基因植株对TuMV的抗性增强,获得了抗病毒病的转基因大白菜植株.
In order to obtain anti-virus transgenic plants of Chinese cabbage, Agrobacterium strain R1000 （pRiA4b） and EHA105 （pTiBo542） which contained a binary vector carrying the TuMV-cp gene were used for genetic transformation. PCR and PCR-Southern analysis of these plants showed that they produced positive hybridization, indicating that the TuMV-cp gene has been introduced and integrated into genome of these plants. RT-PCR analysis proved that the TuMV-cp gene acquired stable expression in transgenic Chinese cabbage. Molecular detections of the transgenic plants of T1 generation proved that TuMV-cp gene inheritance was stable in the progeny. The virus inoculation experiment of TuMV showed that both the disease incidence and the disease index of transgenic T1 generation were lower than those of the non - transgenic plants. These data demonstrated that anti-virus transgenic plants were obtained.
出处
《哈尔滨工业大学学报》
EI
CAS
CSCD
北大核心
2006年第5期793-796,共4页
Journal of Harbin Institute of Technology
