摘要
目的:探讨电针对缺血性脑卒中的作用机制。方法:线栓法制备Wistar大鼠局灶脑缺血再灌注模型。将大鼠随机分为正常组、模型组、电针组。选取双侧“合谷”穴为电针穴位,采用原位杂交法检测血管内皮生长因子(VEGF)mRNA的表达,免疫组化法检测血管生成素(Ang-1)和内皮抑素(Endostatin)蛋白的表达。结果:模型组VEGFmRNA、Ang-1蛋白、Endostatin蛋白的表达较正常组增加(P<0·05),电针组VEGFmRNA、Ang-1蛋白比模型组增加更明显(P<0·05),而En-dostatin蛋白表达增加较模型组明显下降,差异有显著性意义(P<0·05)。结论:电针可能通过上调血管生长因子和下调血管抑制因子的表达来促进局灶脑缺血再灌注后的血管新生。
Objective To explore the mechanism of electroacupuncture （EA） in improving ischemic stroke. Methods A Wistar rat model of focal cerebral ischemia reperfusion was made by filament occlusion. The rats were randomly divided into a normal group, a model group, an EA group. EA was given at bilateral ＂Hegu＂ （LI 4） in the EA group. Vascular endothelial growth factor （VEGF） mRNA was detected with in situ hybridization and expression of angiogenin-1 （Ang-1） and endostatin proteins with immunohistochemical method. Results The expressions of angiogenic growth factors including VEGF and Ang-1 in the EA group were significantly increased, while the expressions of endostatin was significantly decreased as compared with those in the model group （both P〈0. 05）. Conclusion EA improving ischemic stroke is carried out possibly through up-regulating the expression of angiogenic growth factors and down-regulating the expression of antiangiogenic growth factors.
出处
《中国针灸》
CAS
CSCD
北大核心
2007年第2期129-133,共5页
Chinese Acupuncture & Moxibustion
基金
重庆市科委攻关项目:渝科发计字2003-7
重庆市卫生局中医药科研项目:渝中医2005-B-24
