摘要
目的 探讨电针促进局灶性脑缺血再灌注大鼠脑内缺血区血管再生的作用机制.方法 选择Sprague-Dawley大鼠84只,分为对照组、模型组和模型电针组.采用线栓法制备局灶性脑缺血再灌注模型并按局灶性脑缺血1h再灌注后观察时间点,将模型组和模型电针组分为第1,3,7,14和21天5个亚组.取双侧合谷穴（LI4）为电针刺激穴位.再灌注第3,7,14天,采用逆转录聚合酶链反应法检测基质细胞衍生因子-1α（SDF-1α）mRNA的表达;再灌注后各时间点,采用免疫组织化学法检测SDF-1α蛋白的表达,CD34标记微血管并计数.结果 与对照组各时间点比较,模型组、模型电针组缺血区大脑皮质SDF-1α蛋白表达增加（P＜0.05）;与模型组比较,再灌注第3,7,14天,模型电针组缺血区大脑皮质SDF-1α mRNA表达增加（P＜0.05）.再灌注第1天,模型电针组缺血区大脑皮质SDF-1α蛋白表达增强,微血管计数增加,但与模型组比较,差异无统计学意义（P＞0.05）.再灌注第3,7,14,21天,模型电针组缺血区大脑皮质SDF-1α蛋白表达明显增强,微血管计数明显增加（P＜0.05）.结论 电针可能通过上调局灶性脑缺血再灌注大鼠缺血区大脑皮质SDF-1α mRNA及蛋白质的表达而促进缺血区大脑皮质血管再生.
Objective To investigate the mechanism by which electro-acupuncture （EA） promotes revascularization in the brain after focal cerebral ischemia and reperfusion.Methods The Sprague-Dawley rat model of focal cerebral ischemia was made by filament occlusion. The rats were randomly divided into a control group, a model group, and an EA group. The model and EA groups were each divided into 5 subgroups receiving reperfusion 1, 3,7, 14 or 21 days after ischemia. EA was given at the bilateral Hegn point （LI 4） in the EA group. The expression of stromal cell-derived factor-1α（SDF-1α） mRNA was detected using a RT-PCR in the 3, 7 and 14 day subgroups.The immunohistochemical method was employed to detect the expression of SDF-1α protein. Results Compared with the control group, expression of SDF-1α protein increased significantly in the model and EA groups. Compared with the model group, the expression of SDF-1α mRNA increased significantly in the 3, 7 and 14 day subgroups.SDF-1α protein expression and microvessel count increased slightly but not significantly in the 1d subgroup, but the increases were significant in the 3, 7, 14 and 21 day subgroups.Conclusions EA may promote angiogenesis in an ischemic area of the cortex by increasing the expression of SDF-1αmRNA and its protein after focal cerebral ischemia and reperfusion.
出处
《中华物理医学与康复杂志》
CAS
CSCD
北大核心
2010年第6期409-413,共5页
Chinese Journal of Physical Medicine and Rehabilitation
基金
重庆市卫生局中医药科研项目（渝中医2005-B-24）,高等学校博士学科点专项科研基金（20095503110001）
关键词
电针
局灶性脑缺血/再灌注
基质细胞衍生因子-1
血管再生
合谷穴
Electro-acupuncture
Focal cerebral ischemia,reperfusion
Stromal cell-derived factor-1
Vascular regeneration
Hegu point
