摘要
为了探讨仅含有BH3结构域的促凋亡蛋白BclGs的促凋亡机理,从人睾丸cDNA文库中克隆BclGs基因编码区,将其与pEGFP-C1载体连接,构建载体pEGFP-BclGs,转染COS7细胞和多种肿瘤细胞(如Hela、HepG2和MCF7等),研究BclGs对肿瘤细胞的促凋亡功能。结果表明,pEGFP-BclGs可以诱导不同的肿瘤细胞凋亡,但凋亡率存在差别;BclGs在不同细胞的线粒体中均呈点状分布。线粒体是诱导肿瘤细胞凋亡的有效细胞器,BclGs在线粒体中呈点状分布。
In order to investigate the molecular mechanism of BH3-only proapoptotic BclGs inducing apoptosis,BclGs gene was cloned from human testis cDNA library and constructed into the expressing vector pEGFP-C1. The fusion protein pGFP-BclGs was successfully detected by fluorescent microscope analysis and Western blotting. The result demonstrated that BclGs could induce several tumor cells apoptosis, but showed difference in apoptosis rate. Therefore ,the conclusion can be drawn that the plastochondria was an effective cell organ which can induce the tumor cell to apoptosis,and BclGs assumes the punctual distribution in plastochondria.
出处
《西北农林科技大学学报（自然科学版）》
CSCD
北大核心
2007年第3期29-32,37,共5页
Journal of Northwest A&F University(Natural Science Edition)
基金
国家自然科学基金项目(30200043)
