摘要
目的观察不同方式修复猪Ⅲ度烧伤阴囊对睾丸生精功能的影响。 方法将20只雄性贵州小型香猪按随机数字表法分为正常对照组、自然愈合组、皮瓣修复组、植皮修复组，每组为5只。正常对照组不致伤，将后3组猪阴囊制成Ⅲ度烧伤。白然愈合组创面不行特殊处理，任其白行愈合；皮瓣修复组取腹股沟皮瓣修复创面；植皮修复组取下腹部全厚皮修复创面。观察后3组猪伤后即刻及伤（术）后3个月阴囊外观；伤（术）后3个月，取后3组猪双侧睾丸标本，原位末端标记法检测生精细胞凋亡情况，免疫组织化学法检测b cl-2蛋白表达。同法观察或检测正常对照组猪以上指标。对数据行单凶素方差分析及LSD检验。 结果 （1）正常对照组猪阴囊皮肤有皱褶，具备收缩功能。其余3组猪阴囊烧伤后即刻呈皮革样改变，质硬。伤（术）后3个月，白然愈合组创面呈瘢痕愈合，睾丸被挤入腹股沟区；皮瓣修复组重建阴囊皮肤较厚，睾丸位于皮瓣内；植皮修复组重建阴囊皮肤薄，睾丸位于阴囊内。（2）正常对照组各级生精细胞排列规则，少量精母细胞和精子细胞凋亡；自然愈合组、皮瓣修复组、植皮修复组生精上皮变薄，大部分生精细胞凋亡，以精原细胞和精母细胞为主。自然愈合组、皮瓣修复组、植庋修复组、正常对照组生精细胞凋亡指数分别为（46.3±3.3）%、（40.9±3.5）%、（20.6±2.3）%、（7.5±1.9）%，组间比较差异有统计学意义（F = 405. 65，P〈0．01）。前3组组间两两比较，差异均有统计学意义（P值均小于0．01）。（3）自然愈合组、皮瓣修复组、植皮修复组、正常对照组的b cl-2蛋白表达水平分别为（52±5）%、（53±4）%、（64±5）%、（75±5）%，组间比较差异有统计学意义（F = 56. 63，P〈0．01）。自然愈合组与皮瓣修复组该指标水平相近（P=0.66），均显著低于植皮修复组（P值均小于0．01）。 结论 无论瘢痕化愈合还是用皮瓣移植或者植皮方式修复猪阴囊Ⅲ度烧伤创面，对生精功能均有抑制作用，其中植皮修复造成的影响相对较小。
Objective To observe the effects of different ways in repairing scrotum of pigs with fullthickness burn on spermatogenesis of testis. Methods Twenty male Guizhou miniature pigs were divided into normal control （NC）, natural-healing （NH）, flap-repairing （FR）, and skin-grafting （SG） groups according to the random number table, with 5 pigs in each group. Pigs in NC group were not subjeeted to any injury. Scrotum of pigs in the latter three groups were inflicted with full-thickness burn. Wounds in NH group healed naturally. Wounds in FR group were repaired with inguinal region flap, and those in SG group with full-thickness skin from lower abdomen. Appearance of scrotum in the latter three groups was observed right after injury, and three months post injury or surgery （ PIM or PSM）. Specimens of testes of pigs in the latter three groups were obtained in PIM or PSM 3 to detect apoptosis of spermatogenic cells with TUNEI., and bcl-2 protein expression with immunohistochemistry. The same indexes were observed and determined in pigs of NC group. Data were processed with une-way analysis of variance and LSD test. Results （ 1 ） Scrotum of pigs in NC group had skin iolds with contraction function. Scrotum of pigs became hard with a leathery appearance right after burn in the other three groups. In PIM or PSM 3, wounds of pigs in NH group healed with scar, and the testes were squeezed into inguinal region. Scrotal skin of pigs in FR group was thick with testes in the scrotum, and that of pigs in SG group was thin with testes in the scrotum. （2） Spermatogenic cells in each level in NC group were arranged regularly, with few apoptotic spermatocytes and spermatoblasts. In NH, FR, and SG groups, seminiferous epithelium was thinner with most of the spermatogenic cells showing apoptosis, and they were mainly spermatogonia and spermatocytes. Apoptotic index of spermatogenic cells in NH, FR, SG, and NC groups was respectively （46.3 ± 3.3 ） % , （40.9 4. 3.5） % , （20.6 ± 2.3） % , （7.5 ± 1.9） % , and the difference among them was statistically significant （ F = 405.65, P 〈 0.01 ）. There were significant statistical differences among the former three groups （with P values below 0.01）. （3） bcl-2 protein expression in NH, FR, SG, and NC groups was respectively （52 ±5）% , （53 ± 4） % , （64 4. 5 ） % , （75 ± 5） % , and the difference among them was statistically significant （ F = 56.63, P 〈0.01）. There was no significant statistical difference in bcl-2 expression between NH group and FR group （ P = 0. 66 ） , and it was lower in both groups as compared with SG group （ with P values below 0.01 ）. Conclusions Either scar healing, flap transplantation, or SG in repairing scrotum with full-thickness burn in pigs inhibits spermatogenesis, but repair with SG produces less deleterious effect on the testis.
出处
《中华烧伤杂志》
CAS
CSCD
北大核心
2012年第2期138-141,共4页
Chinese Journal of Burns
基金
基金项目：贵州省科学技术基金[黔科合J字（2009）2181号]
贵州省卫生厅优秀医学青年科技人才专项科研基金（gzwki2008-2-007）
