摘要
目的探讨缺氧诱导因子-1α（hypoxia．induciblefactor-1α，HIF-1α）与血管内皮生长因子vascular endothelial growth factor，VEGF）在恶性黑色素瘤组织中的表达及其相关性。方法采用免疫组织化学方法研究HIF-1α与VEGF分别在32例恶性黑色素瘤标本与11例健康皮肤标本组织中的表达。结果32例恶性黑色素瘤中HIF-1α的阳性表达率为62．5％（20／32），VEGF的阳性表达率为84．8％（27／32）；11例健康皮肤组织中HIF．1a的阳性表达率为9．1％（1／11），VEGF的阳性表达率为18．2％（2／11）。健康皮肤组与恶性黑色素瘤组间比较，HIF-1α与VEGF的表达差异有统计学意义，且恶性黑色素瘤组的HIF-1α与VEGF的表达呈正相关（P〈0．05）。结论①HIF-1α、VEGF在恶性黑色素瘤中呈高表达，提示其可能参与了恶性黑色素瘤的发生和发展。②HIF-1α、VEGF在恶性黑色素瘤组的表达呈正相关，提示恶性黑色素瘤中的HIF-1α可能参与了对VEGF的调控。
Objective To evaluate the expression of hypoxia-inducible factor-1α（HIF-1α）and vascular endothelial growth factor（VEGF）and their relationship in malignant melanoma. Methods 32 cases of malignant melanoma and 11 samples of normal skin were examined for HIF-la and VEGF expression by immunohistochemistry. Results The expressin rate of HIF-1α and VEGF was 62.5% （20/32） and 84.8%（27/ 32）, respectively, in malignant melanoma, and 9.1% （ 1 / 11 ） and 18.2 % （2/11 ）, respectively, in normal skin, showing a significant difference between the two groups. Positive relationship between the expression of HIF-1α and VEGF was found in malignant melanoma（ P 〈 0.05）. Conclusions （1）The high expression of HIF-1α and VEGF in malignant melanoma indicates that it may play an important role in the generation and development of malignant melanoma. （2）The positive correlation between the expression of HIF-1α and VEGF in malignant melanoma suggests that HIF-1α may be involved in the regulation of VEGF in malignant melanoma.
出处
《中华整形外科杂志》
CAS
CSCD
北大核心
2009年第2期134-136,共3页
Chinese Journal of Plastic Surgery
基金
基金项目：湖南省自然科学基金资助项目（07JJ4005）
