摘要
脱氧鬼臼毒素是砂地柏Sabina vulgarisAnt.中的主要杀虫活性成分之一。为探讨其作用机理,以叶碟饲喂法处理粘虫MythimnaseparataWalker4龄幼虫,测定了饲喂处理12h、24h、36h、48h和72h后试虫的羧酸酯酶(CarE)、酸性磷酸酯酶(ACP)、碱性磷酸酯酶(AKP)、谷胱甘肽S-转移酶(GSTs)和多功能氧化酶(MFO)的活性。结果表明:脱氧鬼臼毒素对粘虫羧酸酯酶的活性无明显影响;对酸性磷酸酯酶具有明显的抑制作用,且随着处理时间的延长,抑制作用增强;对碱性磷酸酯酶的影响较为复杂,表现为先抑制,后有所恢复,再被抑制的变化过程;对谷胱甘肽S-转移酶处理12h后活性变化不明显,24h后被抑制,36h后逐渐激活,与对照差异极显著;对细胞色素P450酶系的O-脱甲基酶活性具有明显的抑制作用。
Deoxypodophyllotoxin is the main insecticidal composition in Sabina vulgaris Ant., an insecticidal plant. In order to study its insecticidal mechanism, the effect of deoxypodophyllotoxin on activities of carboxyl esterases （CarE）, phosphatase, glutathione S-transferase （GSTs） and microsomal mixed function oxidase （MFO） in larvae of Mythimna separata Walker were assayed. The results showed that the activities of CarE in tested larvae had no distinct change; with extending of treatment time, the activities of acid phosphatase （ACP） in tested larvae were gradually inhibited by deoxypodophyllotoxin; the change of alkaline phosphatase （AKP） activity was very complex; the activity of GSTs had no distinct change in 12 h, was inhibited in 24 h, but was activated in 36 h; the O-demethylase activity of cytochrome P450 was inhibited by deoxypodophyllotoxin.
出处
《昆虫学报》
CAS
CSCD
北大核心
2007年第2期186-190,共5页
Acta Entomologica Sinica
基金
国家"十五"科技攻关项目(2004BA516A04)
国家自然科学基金项目(30471156)
关键词
粘虫
砂地柏
脱氧鬼臼毒素
代谢酶
抑制作用
Mythimna separata
Sabina vulgaris
deoxypodophyllotoxin
metabolizing enzymes
inhibition
