摘要
目的了解急性出血坏死性胰腺炎(AHNP)形成过程中血浆内皮素(ET)的水平及其在肾组织内的表达,探讨ET在AHNP肾损害发生中的作用。方法胰胆管内逆行注射5%牛磺胆酸钠(Na-Tc),建立大鼠AHNP模型,用光镜和电镜观察大鼠肾脏病理形态学变化,用放射免疫法测定血浆ET含量,用免疫组织化学染色及计算机图像分析定位、半定量检测ET在肾组织中的表达部位和量的变化。结果大鼠胰胆管内注射Na-Tc3h后,血浆ET显著升高(P<0.05),于12h达峰值,持续到24h仍保持较高水平。同时肾脏出现明显的病理形态学改变,且随着时间的延长病变逐渐加重。免疫组织化学染色发现肾小管上皮细胞、肾小球内皮细胞内ET的表达阳性,而且随着术后时间的延长ET免疫组化染色有逐渐增强的趋势。结论ET是AHNP时产生的一种内源性损伤因子,ET在AHNP肾脏损伤的发生、发展中起重要作用。ET升高导致微循环障碍和肾小球系膜细胞增生,可能是AHNP肾损害的发病机制之一。
Objeetive To investigate plasma endothelin levels and the expression of endothelin in the kidneys and determine the role of endothelin in the pathogenesis of renal injury in rats with acute hemorrhagic and necrotic pancreatitis（AHNP）. Methods AHNP was induced in rats by intraductal administration of 5% sodium taurncholate. The concentrations of endothelin in plasma were determined with radioimmunoassay. The pathologic morphologic changes of kidney were observed under light microscope and electron microscope. The expression of endothelin in the kidney was determined through immunohistochemistry. Results The concentration of plasma endothelin was elevated 3 h after inducement. Compared with the control group, the concentration of plasma endothelin in each AHNP groups were higher and the longer the time after inducement, the higher the concentrations and the more severe pathological alteration of pancreatic and renal tissue in AHNP rats. The positive staining of endothelin was mainly localized at the epithelial cells of tubules and glomerular endothelial cells And along with the development of AHNP, the renal injury was more significant and serious accompanied with the increase of the expression of ET in the renal tissue. Conclusion Our findings suggest that endothelin contributes to the development and aggravation of the renal failure in the course of AHNP. The increase of ET levels can lead to the renal micrecireulatory disturbance and proliferation of mesangial cells, which might be one of the pathogens to induce the renal damage in rats.
出处
《首都医科大学学报》
CAS
2007年第1期56-60,共5页
Journal of Capital Medical University
基金
北京市教委基金(01KG098)资助项目
关键词
急性出血坏死性胰腺炎
肾损害
内皮素
acute hemorrhagic and necrotizing pancreatitis
renal injury
endothelin
