摘要
目的建立HPLC法测定不同产地、不同栽培品系的罗汉果药材中罗汉果苷Ⅴ和11-氧化罗汉果苷Ⅴ。方法采用ODS（250mm×4．6mm，5μm）色谱柱，以乙腈-水为流动相进行梯度洗脱，体积流量：0．75mL／min，检测波长：210nm，柱温：40℃。结果罗汉果苷Ⅴ在0．8046～20．1150μg线性关系良好（r=0．9998），平均回收率为104．6％，RSD为3．28％（n=6）。11-氧化罗汉果苷Ⅴ在0．5985～14．9625μg线性关系良好（r=0．9984）。结论本方法简便、快速、准确，可作为罗汉果药材的质量控制方法。
Objective To establish an optimal HPLC method for determining the contents of mogroside Ⅴ and 11-oxomogroside Ⅴ in the dried mature fruits of Momordicae grosvenori from various habitats and breeds. Methods ODS Column （250 mm × 4. 6 mm, 5 μm） was used with acetonitrile-water as mobile phase in a gradient program, flow rate was 0.75 mL/min, detection wavelength was set at 210 nm, column temperature was 40℃. Results The liner range of mogroside Ⅴ was 0.804 6-20. 115 0 μg （r=0. 999 8 ） and the average recovery of mogroside Ⅴ was 104.6%, RSD was 3.28% （n=6）. The liner rangeof 11-oxomogroside Ⅴ was 0. 598 5-14. 962 5 μg （r=0. 998 4） and the average recovery was 102.5%, RSD was 4.43% （n=6）. Conclusion HPLC method is simple, rapid and accurate. It can be used for the quality control of the dried mature fruits of M. grosvenori.
出处
《中草药》
CAS
CSCD
北大核心
2007年第2期196-198,共3页
Chinese Traditional and Herbal Drugs
基金
国家"863"资助项目(2004AA2Z3730-19)
关键词
罗汉果
罗汉果苷Ⅴ
11-氧化罗汉果苷Ⅴ
高效液相色谱
the dried mature fruits of Momordica grosvenori Swingle
mogroside Ⅴ
11-oxomogroside Ⅴ
HPLC
