摘要
目的探讨孕妇血清中乙型肝炎病毒(HBV)核心抗原(HBeAg)、前S1抗原(Pre-S1Ag)和病毒DNA(HBV-DNA)与胎儿宫内乙型肝炎病毒感染的关系。方法应用酶联免疫吸附试验(ELISA)和荧光定量聚合酶链反应(FQ-PCR)对165例乙肝表面抗原(HBsAg)阳性孕妇的母血及产后婴儿外周血的乙型肝炎血清学标志物(HBV-M)和HBV-DNA进行检测。结果165例HBsAg阳性孕妇血清中HBeAg、Pre-S1Ag和HBV-DNA的检出率分别为29.1%、31.5%和38.2%,所生婴儿外周血中HBsAg和HBV-DNA的检出率分别为7.3%(12/165)和23.6%。HBeAg、HBVPre-S1Ag和HBV-DNA阳性孕妇所生婴儿外周血HBV-DNA的检出率分别为79.2%、75.0%和66.7%,显著高于HBeAg、Pre-S1Ag和HBV-DNA阴性者的5.1%、6.2%和0.0%,(P<0.01)。孕妇血清中平均HBVDNA的含量为(7.32±1.79)(拷贝数/mL的对数),显著高于婴儿外周血HBVDNA的(5.86±1.33),(P<0.01),婴儿外周血中HBVDNA阳性率随孕妇HBVDNA含量增加而显著增加,且呈正相关(r=0.43,P<0.01)。结论孕妇血清中HBeAg、Pre-S1Ag和HBV-DNA阳性是胎儿宫内感染HBV的高危因素,胎儿宫内感染率随母血中HBV-DNA含量增加而增加。
[Objective] To investigate the relationship between intrauterine infection and HBeAg, Pre-S1Ag and HBV-DNA in pregnant women with chronic Hepatitis B. [Methods] Serologic HBV marks （HBVM） and HBV-DNA in blood samples of 165 HBsAg（＋） pregnant women, peripheral blood of neonates were detected by enzyme linked immunoadsorbent assay （ELISA） and flurence quantitative polymerase chain reaction （FQ-PCR）. [Results] The positive rate of HBeAg, Pre-S1Ag and HBV-DNA in HBsAg（＋） pregnant women were 29.1%, 31.5% and 38.2%, respectively. The positive rate of HBsAg and HBV-DNA in peripheral blood of neonates were 7.3% and 23.6% respectively. When HBeAg（＋）, Pre-S1Ag（＋） and HBV- DNA（＋） in pregnant women, HBV-DNA positive rate of their infants blood was 79.2%, 75.0% and 66.7%, respectively, which was significantly higher than those with HBeAg（-）, Pre-S1Ag（-） or HBV-DNA（-） （P 〈0.01）. The average concentrations of serum HBV-DNA in 48 pregenant women was （7.32±1.79）, which was significantly higher than that of peripheral blood of neonates （5.86±1.33） （P 〈0.01）. And their HBV-DNA load was positively correlated （r=0.43, P 〈0.01）. [Conclusion] HBeAg（＋）, Pre-S1Ag（＋） or HBV- DNA（＋） in pregnant women is one of the high risk factors of fetal intrauterine infection. Intrauterine infectious rate increased significantly when HBV DNA load was increased in blood of pregnant women.
出处
《中国现代医学杂志》
CAS
CSCD
北大核心
2007年第3期360-362,364,共4页
China Journal of Modern Medicine
关键词
肝炎病毒
乙型
病毒复制
疾病传播
垂直
hepatitis B virus
viral replication
disease transmission
vertical
