摘要
目的探讨载脂蛋白E（ApoE）基因多态性与2型糖尿病合并冠心病的关系。方法采用以医院为基础的配对病例对照研究方法，对100对研究对象进行了研究。应用聚合酶链式反应-限制性片断长度多态性（PCR—RFLP）技术进行ApoE基因多态性分析。结果ApoE基因多态性与2型糖尿病合并冠心病之间存在统计学关联，携带ApoEε4等位基因的2型糖尿病患者合并冠心病的危险性高于不携带ApoEε4等位基因的患者，OR及其95％ CI 分别为2．800、1．360～5．764，经糖尿病病程调整后此种关联仍然存在。未发现ApoEε2和ε3等位基因与2型糖尿病合并冠心病之间有统计学关联。结论携带ApoE基因ε4等位基因增加2型糖尿病合并冠心病的危险性。
Objective To explore the association between polymorphism of apolipoprotein E （ApoE） gene and coronary heart disease with type 2 diabetes patients. Methods A hospital-based 1 ： 1 matched case-control study was conducted, in which 100 pairs of subjects were recruited. Polyrnorphism of ApoE gene was analyzed by polymerase chain reaction （PCR） and restriction fragment length polymorphism （RFLP）. Results The ε4 allele of ApoE gene was associated with development of coronary heart disease with type 2 diabetes patients, the ε4 allele of ApoE gene was more frequent among the patients of coronary heart disease with type 2 diabetes patients. Compared with type 2 diabetes patients without the allele of ApoE gene, the OR with 95% CI of type 2 diabetes patients with the ε4 allele of ApoE gene was 2. 800 （ 1. 360- 5. 764）. After adjusting for diabetes duration, the result still showed that the association between the ε4 allele of ApoE gene and coronary heart disease with type 2 diabetes patients was statistically significant. No associations were observed between the ε2 and ε3 alleles of ApoE gene and development of coronary heart disease with type 2 diabetes patients. Conclusions ε4 allele of ApoE gene is associated with higher risk for the development of coronary heart disease with type 2 diabetes patients.
出处
《疾病控制杂志》
2007年第1期27-29,共3页
Chinese Journal of Disease Control and Prevention
基金
天津市高等学校科技发展基金项目(20050104)1
关键词
载脂蛋白E类
糖尿病
2型
冠状动脉疾病
Apolipoproteins E
Diabetes mellitus, type 2
Coronary disease
