摘要
应用生物素化菜豆凝集素（Biotin－PHA）作为探针，对人体胃癌66例，正常胃粘膜10例，异型增生30例，进行亲合组织化学法研究，同时应用图像分析技术定量测定异型增生及胃癌细胞表面菜豆凝集素受体含量，结果以平均光密度值MOD表示。研究发现正常胃粘膜PHA受体阴性，PHA受体的分布与胃癌组织学类型及分化程度有关。MOD值按轻增、中增、重增及胃癌的顺序而递增，各级间有显著性差异。图像分析技术有利于异型增生分级及其与胃癌的鉴别诊断。
Biotin -PHA was used to study 66 caes of gastric carcinoma, 10 cases of normal gastric mucosa . arid 30 cases of dysplasia by using the affinity histochemical method. The average relative amounts of PHA lectin binding were measured (luantitatively by the lmage analysis technique in dysplasia and gastric carcinoma. The results showed that no PHA receptors were found in the normal gastric mucosa. The distribution of PHA receptors is relative to the histologic type and the degree of differentiation of gastric carcinoma. MOD shows progressively increase in the cases of mild dysplasia, moderate dysplasia, severe dysplasia and gastric carcinoma. The statistical analysis showed a significant difference between the above four groups (P<0.001). The image analysis technique therefore is useful in the classification of dysplasia and for distinguishing between dysplasia and gastric carcinoma.
出处
《合肥医学院学报》
1996年第3期170-173,362,共4页
Journal of Zunyi Medical University
关键词
异型增生
凝集素
图像分析
肿瘤
gastric carcinoma dysplasia lectin image analysis technique
