摘要
本文应用ＳＤＳ－ＰＡＧＥ，蛋白质免疫印渍技术及以凝集素为探针对一侧输卵管结扎兔着床期子宫内膜糖蛋白进行了研究。结果表明１．ｃｏｎＡ－ｂｉｎｄｉｎｇＧＰｓｏｓ非着床侧略高于着床侧。２．ＷＧＡ－ｂｉｎｄｉｎｇＧＰ着床侧各条带扫描后峰值均高于非着床侧。３．分子量为９７．３ＫｄＰＮＡ－ｂｉｎｄｉｎｇＧＰｗ和８５．９ＫｄＰＮＡ－ｂｉｎｄｉｎｇＧＰＳＤＳ着床侧明显高于非着床侧，而３８．５ＫｄＰＮＡ－ｂｉｎｄｉｎｇＧＰＳＤＳ非着床侧则明显高于着床侧，４．分子量为９７．３Ｋｄ和ｇｏ．６ＫｄＲＣＡ－ｂｉｎｄｉｎｇＧＰＳＤＳ着床例明显高于非着床侧，而３７．２ＫｄＲＣＡ－ｂｉｎｄｉｎｇＧＰＳＤＳ非着床例明显高于着床侧．显示在同一激素状态下，胚泡着床与否影响到子宫内膜糖蛋白出现了不同变化。
The effect of glycoprotein embryo endometrium in implantation stage of one side eviduct ligated rabbit was studied by using SDS-PAGE,Western blotting and lectins.The results show that1.the ConA-binding GPSDS is slightly higher in no embryo implanting endometrium than in embryo implanting one; 2.the scanning peak area of every band of WGA-binding GP iS higher evidently in embryo implantation endometrium than in no embryo endometrium ;3. the peak area of 97. 3Kd PNA-binding GPW and 85. 9Kd PNA-binding GPSDS is higher in embryo implantation endometrium than in no embryo endometrium,but the peak area of 75. 5Kd PNA-binding GPSDS is higher in no embryo endometrium than in embryo implantation endometrium ; 4. the peak area of 97. 3Kd and 90. 3Kd RCA-binding GPSDS is higher in embryo implantation endometrium than in no embryo endometrium,the peak area of 37. 2Kd RCA-binding GPSDS is higher in no embryo endometrium than in embryo implantation endometrium. It shows that the implantation of embryowill cause different changes for the glycoprotein of endometrium under the same level of hormone.
出处
《生殖与避孕》
CAS
CSCD
北大核心
1994年第6期422-426,共5页
Reproduction and Contraception
