摘要
目的探讨母体孕期染铝对子代脑神经发育的毒性作用。方法 4~5月龄SD大鼠,雌雄各30只,1∶1合笼喂养,每日早晨查雌鼠阴栓,发现阴栓即进入实验（E0）,将孕鼠随机平分为2组。染铝组E0~E18期间以三氯化铝100 mg.kg-1.d-1灌胃;正常对照组以等量蒸馏水灌胃。分别取孕鼠两个年龄段子代即生后继续喂养的1月子代及3月子代鼠脑组织,每只孕鼠任取其子代2只,每小组子代30只。常规石蜡切片,经神经元特异烯醇酶（NSE）免疫组化染色,分别对大脑皮质Par1区内锥体细胞层NSE免疫组化染色阳性反应细胞进行计数,并用细胞形态学计量方法测量阳性反应产物的平均光密度。结果染铝组子代NSE免疫组化染色阳性细胞均明显少于对照组子代（P〈0.05）,阳性反应产物平均光密度均低于对照组子代（P〈0.05）。结论大鼠孕期染铝可导致子代鼠脑神经元数量减少,从而导致子代鼠神经发育障碍。
Objective To investigate the toxic effect of aluminum on cerebral cortex neurons in offsprings of pregnant rats.MethodsTotally 60 Sprague-Dawley rats（30 male and 30 female） at an age of 4-5 months were employed and mated according to the proportion of 1∶1.The female rats which were discovered to have vaginal plug were enrolled into the experiment.The pregnant rats were randomly divided into two groups equally：aluminum exposed group（AlCl3 100 mg·kg-1·d-1 was administrated intragastrically from EO to El8） and control group（equal amount of distilled water was used）.The offsprings of the pregnant rats were divided into 2 groups：1 month group and 3 month group（2 offsprings from 1 pregnant rat and 30 offsprings in each group）.The brains of the offsprings were removed to do traditional parafin section preparation and immunohistochemical staining through neuron specific enolase（NSE）.NSE-positive cells in Par1 layer of cerebral cortex were counted and their average optical densities were analyzed with cell morphometric technique.Results The numbers of NSE positive cells were obviously fewer in aluminum exposed group than those in control group（P〈0.05）;the average optical densities were obviously lower in aluminum exposed group than those in control group（P〈0.05）.Conclusion Exposure to aluminum during pregnancy can decrease the numbers of neurons in offspring rats,which may induce neurodevelopmental disorder.
出处
《局解手术学杂志》
2011年第2期142-144,共3页
Journal of Regional Anatomy and Operative Surgery
基金
贵州省教育厅自然科学研究项目(黔教科2007-031)
关键词
孕期
铝
脑
神经元
子代
pregnancy
aluminium
brain
neuron
offspring
