摘要
试验采用组织分离法分离花生褐斑病病菌,研究了病菌的培养特性,采用离体叶片接种和整株接种鉴定法对分离病菌的致病性进行了检测,并对感病植株的叶片进行病菌再分离鉴定。结果表明:菌丝生长和产孢适宜温度为15￣30℃,最适为25℃,低于5℃或高于40℃病菌停止生长和产孢;近紫外照射能显著提高病菌的产孢量。两种接种方法均能使叶片和植株感病,由接种感病叶片再分离的病菌符合花生褐斑病的病原特点。因此,本研究获得了致病力强的花生褐斑病致病菌。
The pathogenic fungi in peanut early leaf spot disease were isolated by tissue isolating method. The affecting factors on the mycelium and sporulation of this pathogenic fungi were investigated. And the pathogenic power was also investigated by leaf in vitro inoculation and spraying total plant with sporule suspension. The result showed that the pathogenic fungi could grow and sporulate between 15-30℃, and the best suitable temperature was 25℃. In addition, using black light could accelerate sporulation. The pathogenesis experiments showed that this fungus could infect peanut, and pathogenic fungi was Cercospora arachidicola Hori.
出处
《中国农学通报》
CSCD
2007年第3期343-346,共4页
Chinese Agricultural Science Bulletin
基金
国家科技成果重点推广计划"优质高产专用型花生新品种丰花3号
丰花4号及配套技术"(2004EC000174)
关键词
花生
褐斑病
病原真菌
致病性
Peanut, Early leaf spot disease, Pathogenic fungi, Isolation, Pathogenic power
