摘要
目的 :探讨炎症细胞趋化因子单核细胞趋化蛋白 1 (MCP- 1 )在脊髓损伤早期的表达变化及其在脊髓继发性损伤炎症免疫反应中的作用机制。方法 :采用改良 Nystrom法制备大鼠胸段脊髓后路压迫损伤模型 ,运用 RT- PCR技术检测脊髓损伤后 6 h的 MCP- 1 m RNA的表达水平 ,免疫组化方法检测损伤后 3、6、1 2 h和 1、3、5、7d各个时间段 MCP- 1阳性细胞与损伤局部单核 /巨噬细胞数目的变化。 结果 :脊髓损伤后 ,MCP- 1 m RNA水平明显升高 ,MCP- 1阳性细胞高峰时间出现在脊髓损伤后 1 2 h至 3d,同时 ,单核 /巨噬细胞高峰时间出现在损伤后 3～ 7d。 结论 :MCP- 1、单核 /巨噬细胞均参与了脊髓损伤后早期的继发反应 ,单核 /巨噬细胞出现的高峰时间滞后于 MCP-
Objective:To study the expression level of MCP-1mRNA after spinal cord injury(SCI)and its role in the secondary immunoreaction at the early stage after SCI.Methods:The animal model of Nystro m's posterior compression in spinal cord was adopted in this study.The expression level of MCP-1mRNA was detedted by RT-PCR on6h post-injury.The expression and distribution of MCP-1positive cells and the infiltration of monocytes/macrophages were measured by im-munohistochemistry on3h,6h,12h,1d,3d,5d,7d post-injury.Results:The level of MCP-1expression significantly in-creased after SCI and the peak MCP-1positive cells appeared at the early period after SCI(12h-3d post-injury),while the peaks of monocytes/macrophages ocurred3-7d post-injury.Conclusion:Both MCP-1and monocyte/macrophage take part in the secondary immunoreaction at the early stage after SCI.In the local injury site,the peak of monocytes/macrophages lags behind that of MCP-1positive cells.
出处
《第二军医大学学报》
CAS
CSCD
北大核心
2004年第7期766-768,共3页
Academic Journal of Second Military Medical University
