摘要
本文提出了一种结合酪胺聚合膜和纳米金为界面的甲状腺激素压电免疫传感器.先在晶振表面电聚合一层酪胺聚合膜,然后通过聚酪胺一端的氨基和纳米金之间的作用力在聚合膜表面自组装一层纳米金,再在纳米金上固定T4抗体,实现对T4的检测.考察了一些实验条件,如免疫反应时间,抗体浓度等对传感器响应性能的影响,该传感器对T4在1.0～97.7μg/dL范围具有很好的线性响应,并将此传感器与采用戊二醛交联法和半胱氨吸附界面的传感器进行了比较.
A new QCM immunosensor based on self-assembled gold nanoparticles to the electropolymerized polytyramine （Pty） film was developed for the detection of thyroxine （T4）. A Pty film was firstly electro- polymerized on the surface of the QCM and subsequently a self-assembled nano-gold layer was applied on the Pty film, in order to immobilize anti-T4 antibodies. Finally, through the immunoreaction of anti-T4 antibodies with T4, the detection of T4 was realized. A series of experimental conditions were optimized including immunoreaction time, concentration of anti-T4 antibody, etc. The immunosensor showed a good linear response towards T4 in a concentration range of 1.0-97. 7μg/dL. Comparing to the glutaraldehyde binding and Cysteamine methods, the self-assembled gold nanoparticles showed larger frequency response and less non-specific adsorption.
出处
《传感技术学报》
CAS
CSCD
北大核心
2007年第2期254-257,共4页
Chinese Journal of Sensors and Actuators
关键词
QCM
酪胺聚合膜
纳米金
T4
QCM immunosensor
electropolymerized polytyramine
gold nanoparticles
T4
