摘要
目的观察糖尿病大鼠心肌缺血/再灌注后低氧诱导因子-1α(HIF-1α)和血红素氧合酶-1(HO-1)mRAN表达的变化。方法以链脲佐菌素复制糖尿病模型。48只大鼠随机分成非糖尿病假手术(C_S)组、非糖尿病缺血/再灌注(C_(1/R))组、糖尿病假手术(D_S组和糖尿病缺血/再灌注(D_(1/R))组。检测各组心肌梗死范围、HIF-1α和HO-1 mRAN表达变化。结果C_S组检测不到HIF-1α和HO-1 mRAN表达,D_S组检测到少量HIF-1α和HO-1 mRAN表达；但是糖尿病组心肌缺血/再灌注后HIF-1α和HO-1 mRAN表达低于非糖尿病组(P＜0．01),心肌梗死范围大于非糖尿病组(P＜0．01)。结论糖尿病加重心肌缺血/再灌注损伤,其机制可能与糖尿病心肌缺血/再灌注后HIF-1α和HO-1 mRAN表达相对降低有关。
Objective To study the changes of hypoxia - inducible factor-1α（ HIF-1α） and Heme oxygenase - 1 （ HO - 1 ） mRNA expression induced by myocardial ischemia - reperfusion in diabetic rat heart. Methods The diabetic rat model was made by an intraperitoneal injection of streptozotocin, after 8 weeks, 48 rats were randomly divided into 4 groups ： non - diabetic sham - operated group （ group Cs ） , non - diabetic ischemia - reperfusion group （ group CI/R ） , diabetic sham - operated group （group Ds ） and diabetic ischemia - reperfusion group （group D1/8 ） . Myocardial infarct size, the expression of HIF-1α and HO - 1 mRNA were detected by RT - PCR in each group. Results The expression of HIF-1α mRNA was not appreciable in group Cs, but significantly increased in group C1/R after ischaemia - reperfusion. In group Ds, the expression of HIF-1αcould be detected; however, as compared with group CI/R, the expression of HIF-1α was decreased in group DI/R （P 〈 0.01 ）. The changes in myocardial HO - 1 mRNA expression paralleled those of HIF -1α. Myocardial infarct size was greater in diabetic rats than that in non - diabetic rats （ P 〈 0.01 ）. Conclusion Diabetes increased basal HIF -1α and HO - 1 expression, suggesting a state of pseudohypoxia. These findings show that diabetes aggravated myocardial injury induced by ischemia - reperfusion, which is associated with a relatively reduced expression of the HIF -1α and HO - 1 gene.
出处
《中国急救医学》
CAS
CSCD
北大核心
2007年第1期46-48,共3页
Chinese Journal of Critical Care Medicine
基金
教育部留学回国人员科研启动基金[教外司留(2003)14号]
