摘要
应用原位杂交技术，观察了二乙基亚硝胺（ＤＥＮ）诱发大鼠肝癌前病变组织中胎盘型谷胱甘肽Ｓ转移酶（ＧＳＴ－Ｐ）ｍＲＮＡ的表达。结果显示，ＧＳＴ－ＰｍＲＮＡ主要在癌前病变肝组织中的变异灶及灶外卵圆形细胞内表达，且在变异灶间或和同一灶内阳性细胞间表达程度不尽一致，而正常肝、再生肝组织中未见其表达。
The expression of glutathione S transferase placental (GST P) mRNA was observed in hepatic preneoplastic lesions induced with diethylnitrosamine (DEN) with in situ hybridization in rats. It was found that GST P mRNA mainly expressed in altered foci and some of oval cells in hepatic preneoplastic lesions and the extent of its expression was different among foci or/and positive cells in the same foci whereas no expression was observed in normal and regenerating hepatic tissue. Our findings indicate that cells in altered foci and oval cells may be preneoplastic cells in the experimental hepatocellular carcinoma at the molecular level.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
1996年第6期502-504,共3页
Journal of Third Military Medical University
基金
国家自然科学基金
关键词
肝肿瘤
谷胱甘肽转移酶
MRNA
hepatic preneoplastic lesion
glutathione S transferase placental
in situ hybridization
