摘要
目的 :探讨人膀胱癌多药耐药机制。方法 :应用原位杂交和免疫细胞化学技术 ,分别对人膀胱癌耐药细胞株(BIU 87/ADM)及敏感细胞株(BIU 87)中Bcl 2基因及其产物的表达进行研究。结果 :BIU 87/ADM细胞Bcl 2基因表达呈强阳性( ) ,BIU 87细胞呈弱阳性( +)。结论 :凋亡抑制基因Bcl 2在人膀胱癌多药耐药细胞中的高表达 ,与人膀胱癌多药耐药的产生有关。
Objective: To investigate the mechanism of multidrug resistance in human bladder cancer. Methods: The expression of Bcl 2 gene in cell lines BIU 87/ADM and BIU 87 was detected with in situ hybridization and immunohistochemistry. Results: The expression of Bcl 2 gene was strongly positive in BIU 87/ADM cells but poorly positive in BIU 87 cells. Conclusion: The overexpression of Bcl 2 gene, an apoptotic gene, in BIU 87/ADM cells might contribute to the development of multidrug resistance of human bladder cancer.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
2000年第1期57-59,共3页
Journal of Third Military Medical University
