摘要
为探讨短暂局灶脑缺血后不同灌流期即刻早期基因（ＩＥＧｓ）与凋亡抑制基因在同一脑区的表达状况，采用不开颅血管腔内置线法制作鼠大脑中动脉阻塞（ＭＣＡ０）的局灶缺血再灌流模型，通过免疫组化法观察原癌基因ｃ－ｆｏｓ和ｂｃｌ－２蛋白在缺血３０ｍｉｎ再灌６ｈ和２４ｈ的表达。结果显示∶再灌６ｈ，二者在缺血同侧梨状皮层显著表达，而底节区表达很弱（Ｐ＜０．０１）；再灌２４ｈ，ｃ－ｆｏｓ蛋白在梨状皮层表达显著减弱（Ｐ＜０．０１）；ｂｃｌ－２蛋白表达仍显著（Ｐ＞０．０５），且在底节区的表达有增强（Ｐ＞０．０５）。因而推论∶ｃ－ｆｏｓ与ｂｃｌ－２蛋白的表达可能与缺血损害的内源性保护机制有关。
To study the expression of immediate early genes and apoptosis gene in same cerebral region after transient ischemia followed by varied reperfusion interval,the animal model of focal cerebral ischmia and reperfusion were induced by intraluminal sutrure to occlude the MCA.The expression of cfos and bcl2 gene protein after 30min ischemia and 6h or 24h reperfusion were studied with immunohistochemistry.After 6h reperfusion the expression of cfos and bcl2 protein were significant in piriform cortex, but very weak in basal ganglia (P<0.01);by 24h reperfusion ,cfos protein decreased obviously in piriform while bcl2 protein increased in basal ganglia.The expressions of cfos and bcl2 gene protein probably correlated with the endogenous neuroprotective machenism.
出处
《西安医科大学学报》
CSCD
1999年第2期248-250,共3页
Journal of Xi'an Medical University(Chinese)
基金
陕西省卫生厅科研基金
