摘要
[目的]优化金莲花(Flos Trollii Chinensis)蛋白超声提取工艺,并测定蛋白提取液的抑菌活性。[方法]用L9(34)正交设计优化超声提取金莲花蛋白工艺,用纸片扩散法检测金莲花蛋白抑菌活性。[结果]金莲花蛋白超声提取的最佳工艺为:原料粒度60目,料液比1∶30,提取时间30min。金莲花蛋白提取液(1.25g/L)具有抑制变形杆菌(++)、伤寒杆菌(++)、产气杆菌(+)和大肠杆菌(+)的作用。[结论]利用超声提取方法能够获得高产率的金莲花蛋白,并能保持其生物活性。
[Objective] To optimize the ultrasonic extraction technology for the protein from Flos Trollii Chinensis and determinate the bacteriostatic activity of the protein extract. [Methods] By applThe L9（34） orthogonal test was applied to optimize the ultrasonic extraction technology and the activity of bacteriostasis was determined with slip diffusion method. [Results] The optimized uhrasonic extraction technology was as follows： material granule 60 order, proportion of material to solution 1：30, extracted time 30 min. The extracted protein at 1.25 g/L could inhibit Bacillus proteus（＋＋）, Bacillus typhosus（＋＋）, Bacillus aerogenes（＋）, Bacterium coli（＋）. [Conclusions] The method with uhrasonic extraction can obtain higher plurinatality of protein of Flos Trollii Chinensis without any loss of its biologic activity.
出处
《天津中医药》
CAS
2007年第1期63-65,共3页
Tianjin Journal of Traditional Chinese Medicine
基金
国家教育部重大项目"中药复方功能主治与其药效物质基础的相关性研究"(0176)。
关键词
金莲花蛋白
抑菌活性
提取工艺
protein from Flos Trollii Chinensis
restrain bacterium action
extraction technical
