摘要
目的 探讨癫痫持续状态（SE）后大鼠水通道蛋白-4（AQP4）的表达变化与脑水肿形成之间的关系。方法 54只SD大鼠随机分为对照组（n=6），SE后6h，12h，24h，48h，72h，96h，120h，168h组（n=6）。腹腔注射锂-匹罗卡品建立大鼠SE模型，免疫组织化学染色和逆转录PCR方法检测AQP4蛋白和基因在SE形成后的表达。结果 SE后AQV4蛋白和mRNA24h表达水平明显增加，48h达到高峰，持续72h后下降，168h时仍有表达。SE后AQP4表达变化和脑水肿形成过程在时间上呈明显的正相关（r=0．73，氏0．05）。结论 SE后AOP4表达明显增强，在时间上与脑水肿形成呈正相关，提示AQP4在SE后脑水肿形成过程中起着重要作用。
Objective To investigate the expression of water channel aquapoin-g （AQPg） and its relationship to brain edema formation after status epilepticus （SE） in rats. Methods Fifty-four Sprague-Dawley rats （weighing 250-300 g） were randomly divided into 9 groups （n=6 in each group） the control group and the post-SE 6, 12, 24, 48, 72, 96, 120, 168 h groups. SE models were established with Lithium-pilocarpine. Immunohistochemisn＇y and semi-quantitative reverse transcriptase-polymerase chain reaction （RT-PCR） were used to assess AQPg protein and mRNA expression following SE. Results AQPg expression significantly increased at 24 h, reached to a peak level at 48 h, lasted for 3 d and then began to decline, but still existed at day 7 （P〈0.05 vs control group）. The time-dependent change displayed an obvious positive correlation with the process of brain edema after SE （r=0.73, P〈0.05）. Conclusion The time-related change of AQP4 expression after SE has a positive correlation with brain edema, indicating that AQPg may play an important role in the formation of brain edema following SE.
出处
《中华神经医学杂志》
CAS
CSCD
2007年第2期144-147,共4页
Chinese Journal of Neuromedicine
