摘要
目的 研究大鼠脑出血后水通道蛋白4mRNA表达与血脑屏障通透性的关系。方法 采用自体非抗凝动脉血注入尾状核法制作大鼠脑出血模型，逆转录聚合酶链反应法观察水通道蛋白4mRNA的表达，伊文思兰法检测血脑屏障通透性，干湿重法计算脑含水量以代表脑水肿情况。结果 与对照组比较，脑出血组水通道蛋白4mRNA表达6h即开始升高（1．06±0．12），3天时达到高峰（1．34±0．14），7天时仍高于正常组（P〈0．05）；血脑屏障通透性于出血后6h开始升高（0．5955±0．0956），1天-3天最高（0．8889±0．0968、0．7914±0．0520），5天-7天逐渐降低（P〈0．05）。水通道蛋白4mRNA表达与血脑屏障通透性呈显著正相关（r=0．686，P〈0．01），与脑水肿变化趋势相一致。结论 脑出血后可能通过上调水通道蛋白4mRNA表达，增加血脑屏障通透性，参与脑水肿形成。
Aim To investigate the relafionship between aquaporin-4（AQP4）mRNA expression and blood-brain barrier （BBB） permeability after experimental cerebral hemorrhage in rats. Methods The cerebral hemorrhage models were establisbed by stereotaxic injection of auto-non-anticoagulant artery blood into the caudate nucleus. AQP4 rnRNA expression was detected by RT-PCR, BBB permeability by Evens Blue method and brain water contents by dry-wet weight method. Results In contrast to the control group, AQP4 mRNA expression increased in cerebral hemorrhage group at 6 hours （ 1.06 ± 0.12）, reached peak at 3 days（ 1.34 ± 0.14）, then was still higher than normal at 7 days （ P〈0.05） ; BBB permeability increased at 6 hours after cerebral hemorrhage （0.5955 ± 0.0956）, reached peak at 1-3 days （0.8889 ± 0.0968, 0.7914± 0.0520）, and decreased at 5-7 days gradually （P〈0.05 ）. There was a significantly positive correlation between AQP4 mRNA expression and BBB penncability （r=0.686, P〈0.01）, whieh was comistent with the ehange of eerebral edema. Conclusion Cerebraledema may be formed through the up-regulation of aquapofin-4 mRNA expression and the increase of BBB permeability after cerebral hemorrhage.
出处
《中国动脉硬化杂志》
CAS
CSCD
2006年第4期330-332,共3页
Chinese Journal of Arteriosclerosis
