摘要
目的:建立高效液相色谱法测定复方氯霉素乳膏(新复霜)中氯霉素和醋酸地塞米松的含量。方法:用十八烷基硅烷键合硅胶为填充剂[Symmetry C_(18)柱(4.6 mm×150 mm,5 μm)];以甲醇-水(64∶36)为流动相,流速为0.5 mL·min^(-1);检测波长为240 nm;进样量5 μL。结果:氯霉素及醋酸地塞米松在231.3～809.5μg·mL^(-1)(r=0.9999)和14.1～84.6μg·mL^(-1)(r=0.9999)范围内呈良好的线性关系;氯霉素和醋酸地塞米松精密度 RSD 分别为0.16%和0.22%;平均回收率(n=9)分别为100.1%和99.55%。结论:本方法快速、简便,重现性好,结果可靠,可作为复方氯霉素乳膏的质量控制标准。
Objective：To establish an HPLC method to determine ehloramphenieol and dexamethasone acetate in compound ehloramphenieol creams（ compound new creams）. Method： The HPLC system consisted of C18 column （4. 6 mm × 150 mm,5 μm） with methanol - water （ 64：36 ） as mobile phase, detection at 240 nm, the flow rate was 0. 5 mL · min^-1 and the injection volume was 5μL. Results：The linear range of chloramphenicol and dexamethasone acetate were 231.3 - 809. 5 μg·mL^-1 （ r = 0. 9999） and 14. 1 - 84. 6 μg·mL^-1 （ r = 0. 9999）, the precision RSD were 0. 16% and 0. 22%, and the average recoveries （n = 9）were 100. 1% and 99. 55%. Conclusion： The method is simple, accurate, and reproducible. It＇s very suitable for quality control of the compound chloramphenicol creams.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2007年第1期145-147,共3页
Chinese Journal of Pharmaceutical Analysis
关键词
高效液相色谱法
复方氯霉素乳膏(新复霜)
氯霉素
醋酸地塞米松
含量测定
HPLC
compound chloramphenicol creams （ compound new creams）
chloramphenicol
dexamethasone acetate
content determination
