摘要
目的:建立同时测定复方曲安奈德乳膏中醋酸曲安奈德和盐酸苯海拉明含量的方法。方法:采用反相高效液相色谱法。色谱柱为SunFireC18,流动相为甲醇-水(含三乙胺,磷酸调pH为7.2)(70:30,V/V),流速为1.0ml/min,检测波长为230nm,柱温为30℃。结果:醋酸曲安奈德和盐酸苯海拉明检测质量浓度线性范围分别为0.008～0.048mg/m(lr=0.9990)、0.0484～0.2904mg/m(lr=0.9999);平均回收率分别为99.0%(RSD=1.1%,n=9)、98.8%(RSD=1.6%,n=9)。结论:该方法简便、快速、准确,可用于复方曲安奈德乳膏的质量控制。
OBJECTIVE： To establish a method for the content determination of triamcinolone acetonide and diphenhydramine hydrochloride in Compound triamcinolone acetonide cream. METHODS： RP-HPLC was adopted. The determination was performed on SunFire C18 column with mobile phase consisted of methanol-water （containing triethylamine, pH adjusted to 7.2 using phosphor- ic acid） （70 ： 30, V/V） at the flow rate of 1.0 ml/min. The detection wavelength was set at 230 nm and column temperature was 30 ℃. RESULTS： The linear range of triamcinolone acetonide and diphenhydramine hydrochloride were 0.008-0.048 mg/ml （r= 0.999 0） and 0.048 4-0.290 4 mg/ml （r=0.999 9） with their average recoveries of 99.0% （RSD=1.1%, n=9） and 98.8% （RSD= 1.6%, n=9）. CONCLUSIONS： The method is convenient, rapid, accurate. It can be used for quality control of Compound triam- cinolone acetonide cream.
出处
《中国药房》
CAS
CSCD
2013年第5期446-447,共2页
China Pharmacy
