摘要
以直接加热膨化蔗渣为酶解底物，采用日本Ｙａｋｕｌｔ公司生产的纤维素酶（ＯｎｏｚｕｋＲＳ）进行酶法水解反应，同时开展膜分离实验研究，为该系统优选了膜材料和膜规格，测定了膜分离的有关特性并进行了膜洗涤尝试。实验表明：分割分子量为１．０万的聚醚砜膜对纤维素酶的截留率为１００％；发酵液与膜的吸附对通量的影响不明显，即在发酵液中浸泡过的膜的初始通量与未浸泡过的没多大差别；本实验条件下酶的回收率（以总活性计）只有５６％左右；溶液的ｐＨ值对过滤通量没有影响；液体循环流速对过滤通量的影响表现为：当流速小于２ｍ／ｍｉｎ时，流速对通量无影响，当流速在２～４ｍ／ｍｉｎ的范围内，过滤通量随流速的增加而增大，而当流速大于４ｍ／ｍｉｎ时，流速几乎对过滤通量没影响；膜洗涤实验表明，采用日本Ｈｅｎｋｅ公司生产的膜清洗剂Ｐ３－Ｕｌｔｒａｓｉｌ５６（浓度１％），经约半小时洗涤可使过滤通量回复约９０％。
Direct heating explosion bagasse and the cellulase (Onozuk RS)bought from Yakult Co. Ltd.(Japan)were used in enzymatic hydrolysis and membrane separation experiments. The membrane materials and molecular weight cutoff were selected ;the separation characteristics were determined ,and the washing methods for membrane fouling were also tried. The experimental results indicated that polyether sulfone membrane of MW cutoff of 10 000 had 100% interception rate for the cellulose; the adsorption of the hydrolyszate had no appreciable effect on the flux through the membrane ; the enzyme recovery rate was only 56% through membrane separation in the experimental conditions; the PH of the hydrolyzate had no effect on the flux through the membrane i when the velocity of liquid wae smallen than 2 m/min, the velocity of liquid through the surface of membrane had no effect on the flux through the membrane; when the velocity was 2-4 m/min, the flux increased with the velocity of liquid; and when the velocity was more than 4 m/min,the velocity had no effect on the flux; the membrane being washed with 1% P3-ultrasil 56(bought from Henkel, Japan)can recover 90% of its flux in a washing time of half an hour.
出处
《化学工业与工程》
CAS
1996年第4期30-36,共7页
Chemical Industry and Engineering
关键词
蔗渣
酶法
水解
酶回收
膜分离
纤维素酶
s: enzymatic hydrolysis of sugarcane bagasse recovery of enzyme membrane separation membrane washing
