摘要
目的建立驴胶补血颗粒中黄芪甲苷的含量测定方法。方法采用高效液相色谱（HPLC）法，蒸发光散射检测器，色谱柱采用安捷伦C18（4．6mm×250mm，5μm）柱，乙腈-水（37：63）为流动相，流速为1．0ml，柱温为40℃，漂移管温度105℃；载气流速为2．7ml·min^-1。结果结果表明黄芪甲苷进样量在2．505-12．525μg范围内呈良好的线性关系，相关系数为0．9991，平均回收率为100．1％，RSD为0．54％（n=6）。结论该方法简便，准确，重现性好，可作为该制剂的质量控制。
Objective To establish an HPLC - ELSD method for determination of astragaloside in Lvjiaobuxue Granule. Methods With Agilent XDB C18 （4.6 mm× 250 mm,5 μm） , acetonitrile - water（37： 63 ） was used as the mobile phase, the temperature of drift tube was 105℃. Results The linearity was obtained from 2. 505 - 12. 525 μg（r =0.999 1 ）. The average recovery was 100.1% with RSD =0. 54% （ n = 6 ）. Conclusion The method is sensitive , simple , specific and accurate for determination of astragaloside in Lvjiaobuxue Granule.
出处
《时珍国医国药》
CAS
CSCD
北大核心
2007年第1期98-99,共2页
Lishizhen Medicine and Materia Medica Research
