摘要
目的评价异丙酚对脑缺血再灌注期间沙土鼠缺血去极化的影响。方法雄性沙土鼠24只,体重60-75g,随机分为2组(n=12):对照组和异丙酚组。两组均吸入1％氟烷后气管插管,机械通气,对照组持续吸入1％氟烷,异丙酚组停止吸入氟烷,静脉输注异丙酚1.2mg·kg-1·min-1。股动脉、静脉切开置管,监测平均动脉压(MAP),输液。监测两侧海马CA1区脑皮层直流电位、局部脑血流(rCBF)及直肠温度。1h后,双侧颈总动脉阻断5min,再灌注30min停止吸入氟烷或输入异丙酚。结果缺血即刻两组MAP升高40％,rCBF几乎降至0,再灌注期逐渐恢复至缺血前水平;与对照组比较,异丙酚组脑缺血去极化的起始时间延长(P<0.01),持续时间及恢复时间差异无统计学意义(P> 0.05)。结论异丙酚可延长脑缺血再灌注期间沙土鼠缺血去极化起始时间,而对脑缺血去极化的持续时间及恢复时间无影响。
Objective To evaluate the effects of propofol on iscbemic depolarization during cerebral iscbemia and reperfusion. Methods Twenty-four male gerbils weighing 60-75 g were anesthetized with propofol （ n = 12） or 1% halothane （n = 12）. In pilot study it was demonstrated that propofol infused at 1.2 mg·kg^-1·min^-1 could maintain EEG burst suppression during iscbemia. Nasotracbeal intubation was performed. The animals were mechanically ventilated. Femoral artery and vein were cannulated for MAP monitoring and fluid administration. Rectal temperature, local cerebral blood flow （CBF） and direct current （DC） potentials in bilateral CA1 regions were continuously monitored. Bilateral common carotid arteries were exposed and occluded for 5 min to induce forebrain ischemia. Changes in DC potentials were assessed by measuring onset latency, duration and recovery time of iscbemic depolarization. Results Immediately after ischemia was started MAP was increased by 40% and CBF was decreased almost to 0. During reperfnsion MAP and CBF gradually returned to pre-iscbemic levels. The onset latency of iscbemic depolarization was significantly longer in propofol group than in halothane group （ P 〈 0.01 ）. However there was no significant difference in the duration and recovery time of iscbemic depolarization between propofol and halothane group. Conclusion Propofol prolongs the onset latency of iscbemic depolarization but does not affect duration and recovery time of iscbemic depolarization during cerebral iscbemia and reperfnsion in gerbils.
出处
《中华麻醉学杂志》
CAS
CSCD
北大核心
2006年第12期1098-1100,共3页
Chinese Journal of Anesthesiology
关键词
二异丙酚
再灌注损伤
脑缺血
缺血去极化
Propofol
Reperfusion injury
Brain ischemia
Ischemic depolarization
