摘要
遗传信息由DNA传递至蛋白质,再经蛋白质翻译后糖基化修饰形成糖蛋白。与DNA、蛋白质相比,糖蛋白糖链结构更加多样,功能更加复杂,在一些重大的生理、病理事件中发挥着重要调节作用;而糖链如此复杂的功能是由其多样的结构决定的,糖链结构是糖组学研究的重要内容。本文就近年来糖组学研究中糖蛋白样品的提取分离、糖链释放及结构分析的基本方法及相关技术进展作了简要介绍。
Genetic information is transferred from DNA to protein and the protein is glycosylated subsequently, which results in the forming of glycoprotein. Comparing with DNA and protein, glycoprotein glycan possess more diverse structures and more complex functions which make the glycan play important regnlational roles in many significant physiological and pathological events. And the complex function of glycan attribute to its diverse structure which is one of the important content of glycomics. The recent research progress in analysis techniques and methods of extract and isolate glycoprotein from sample, release the glycan and identify its structure are introduced.
出处
《化学进展》
SCIE
CAS
CSCD
北大核心
2007年第1期130-135,共6页
Progress in Chemistry
基金
国家重点基础研究发展计划(973)项目资助(No.2003CB716400)
关键词
糖组学
系统生物学
糖蛋白
糖链
分离纯化
结构分析
glycomics
system biology
glycoprotein
glycan
isolation and purification
structure study
