摘要
目的:通过观察输精管结扎后前列腺间质的变化以及前列腺细胞凋亡率,探讨输精管结扎对前列腺组织生长的影响及其机制。方法:SD大鼠45只,随机分为A、B、C 3组,每组15只。A组将大鼠双侧输精管结扎;B组皮下注射丙酸睾酮;C组皮下注射生理盐水。1个月后将大鼠处死,取前列腺腹侧叶,切片、HE染色,应用计算机图像分析系统检测各组前列腺间质、上皮及管腔的面积百分率。应用TUNEL法检测各组大鼠前列腺的细胞凋亡。结果:A、B、C组大鼠前列腺组织中间质面积比值分别为:(29.20±6.85)%、(48.90±6.49)%、(39.77±7.58)%,组间比较差异有显著性(P均<0.05)。3组前列腺组织中均有细胞凋亡发生,以上皮组织为主。A、B、C组大鼠前列腺组织细胞凋亡率分别为:(6.39±0.84)%、(2.62±0.57)%、(4.58±0.93)%。A组的细胞凋亡率显著高于B组和C组(P均<0.05)。结论:输精管结扎可诱导大鼠前列腺组织细胞凋亡,可能有助于预防前列腺增生的发生。
Objective ： To detect the effects of vasectomy on the growth and apoptosis of prostatic tissue cells. Methods ： Forty-five SD rats were divided at random into three groups of fifteen each. Vasectomy （Vsm） model was established by ligating the bilateral vas deferens of the rats in Group A and testosterone propionate and normal saline were subcutaneously injected in those of Groups B and C, respectively. The area percentage of each part in the prostatic tissue was measured with computer-assisted image analysis system. The apoptotic rate was examined with TUNEL. Results： The ratio of stromal area to epithelial plus lumen area in the prostatic tissues in Groups A, B and C were （29.20 ±6.85 ）, （39.77 ±7.58 ） and （48.90± 6.49 ）, respectively, and the differences were significant statistically （P all 〈 0. 05 ）. The apoptosis rates in Groups A, B and C were （6.39±0. 84 ） %, （2.62 ±0. 57 ） % and （4.58±0. 93 ） %, respectively, significantly higher in Group A than in Groups B and C （ P all 〈0. 05 ）. Conclusion ： Vasectomy may induce interstitium reduction and cell apoptosis in the prostatic tissues of rats, which may help prevent benign prostatic hyperplasia.
出处
《中华男科学杂志》
CAS
CSCD
2006年第11期1007-1009,共3页
National Journal of Andrology
