摘要
目的:以细胞的贴壁生长机制为着眼点,从组织工程材料中的细胞相容性材料的发展历程出发,对多种聚合物、多种形貌的材料及其产生的细胞效应进行综述。指出从理论上来说,相对于平滑材料表面,多孔结构的材料表面的优越性;若采用生物降解材料可从根本上解决生物相容性问题。资料来源:应用计算机检索PUBMED1997-12/2003-12期间的相关文章,检索词为“tissueengineering,tissueengineeringmaterials,cell-compat-ibility,cellcompatibilitymaterials,processionanchorage”,并限定文章语言种类为English。同时计算机检索万方数据库1997-12/2003-12期间的相关文章,检索词为“组织工程,组织工程材料,细胞相容性,细胞相容性材料,贴壁生长机理”,并限定文章语言种类为中文。资料选择:对资料进行初审,并查看每篇文献后的引文。纳入标准:文章所述内容应与细胞相容性组织工程材料相关。排除标准:重复研究或Meta分析类文章。资料提炼:共收集到41篇相关文献,13篇文献符合纳入标准,排除的28篇文献为内容陈旧或重复。符合纳入标准的13篇文献中,2篇涉及细胞的贴壁生长机制,11篇涉及细胞相容性材料。资料综合:①关于细胞的贴壁生长机制:综述了多种体外培养的细胞具有贴壁生长的特性,并简略描述了细胞在培养用基底材料表面贴壁生长过程。在需要促进细胞生长的场合,构建细胞相容过渡层上应采取更有效的方法以补偿孔洞结构造成的负效应。②关于细胞相容性材料:综述了被研究过的多种聚合物、多种形貌的材料及其产生的细胞效应,指出细胞与不同表面形貌的材料之间的相互作用机制仍是研究工作中的难点,也是非常值得研究的课题。材料表面的物理化学性质和拓扑结构对材料的细胞相容性有着较大影响。细胞与聚合物的相互作用是评价材料细胞相容性的指标。细胞与高分子材料间短期的相互作用程度可由细胞与高分子材料表面的黏附程度来评价,而二者之间长期的相互作用则可以通过检测体外培养细胞的生长情况或在体内植入高分子材料进行评估。结论:从理论上来说,相对于平滑材料表面,多孔结构的材料表面的优越性;若采用生物降解材料可从根本上解决生物相容性问题。
OBJECTIVE： Based on the mechanism of cellular adhesive growth and the developing course of cell compatibility materials, the cytological effects of tissue engineering materials of various polymers and surface topographies are reviewed. And presume theoretically that the materials of porous structure are better than smooth materials in surface, and biodegradation materials can explain the biocompatibility. DATA SOURCES： An online search was conducted in PUBMED database to identify the related articles published from December 1997 to December 2003 with the key words of ＂tissue engineering, tissue engineering materials, cell-compatibility, cell compatibility materials, procession anchorage＂, and the language was limited to English. Meanwhile, the related Chinese articles were retrieved in Wanfang database published at the same period by inputting the key words of ＂tissue engineering, tissue engineering materials, cell-compatibility, cell compatibility materials, mechanism of adhesive growth＂ into computer. STUDY SELECTION＂ All the data were checked primarily, and the quotations of each article were looked up. Inclusive criterion： content related to the cell-compatibility of tissue engineering materials, Exclusion criterion： repeated study or Meta-analysis. DATA EXTRACTION： Totally 41 articles were collected, and 28 ones were deleted due to the repeated or dated contents. Among the 13 articles met the inclusive criterion, 2 ones were about the mechanism of cellular adhesive growth and 11 ones were referred to the cell compatibility materials. DATA SYNTHESIS： ①The mechanism of cellular adhesive growth： To summarize the characteristics of adhesive growth in various in vitro cultured cells, and briefly describe the growth procedure of those cells cultured on substrate materials. Suggesting that compensate for the adverse effects caused by pore space structure, more effective methods should be adopted to form the transition layer of cell compatibility when the cells are required to grow.②Cell compatibility materials： To summarize the cytological effect produced by the materials of various polymers and surface topographies, and point out that the interaction mechanism between cells and materials of different surface topographies is still a difficult but valuable topic in the related fields. The physical and chemical properties as well as topological structure of the materials surface influence on the cell compatibility of materials. The interaction between cells and polymers is the evaluation indicator of cell compatibility of materials. The temporary interaction can be evaluated by the adhesion of cells to polymers surface, while the long-term interaction may be estimated by the growth of in vitro cultured cells or in vivo implanted polymers. CONCLUSION： Theoretically, porous materials show great superiority compared with smooth surface materials; Biodegradation materials can tackle the biocompatibility completely.
出处
《中国临床康复》
CSCD
北大核心
2006年第45期225-227,共3页
Chinese Journal of Clinical Rehabilitation
