摘要
本文研究了黄腐酸钠对大鼠卵巢颗粒细胞和肾上腺皮质细胞分泌功能的影响。在细胞培养液中加入不同浓度的黄腐酸钠（１～１００μｇ／Ｌ），共育３ｈ后用放射免疫分析法测定培养液中雌二醇和皮质酮含量的变化。结果表明黄腐酸钠（１０～１００μｇ／Ｌ）可明显刺激大鼠卵巢颗粒细胞分泌雌二醇（Ｐ＜０．０１）；对大鼠肾上腺皮质细胞分泌皮质酮的影响较复杂，只在４μｇ／Ｌ时有刺激分泌作用（Ｐ＜０．０５），低剂量（１μｇ／Ｌ）和高剂量（１０～１００μｇ／Ｌ）均无作用。
Effects of Sodium Fuliate (SF) on estradiol (E 2) and corticosterone production were studied in cultured rat granulosa and adrenal cortical cells. SF (1￣100μg/L) was added to cultured cells, and after 3h of incubation the quantity of E 2 and corticosterone was determined using radioimmunoassay. The results showed that SF (10￣100μg/L) could promote E 2 production (P<0.01),and had a complex effect on the secretion of adrenal cortical cells of rat. SF only 4μg/L exerted a slightly stimulatory effect on corticosterone production (P<0.05),both lower dosage (1μg/L) and higher dosage (10￣100μg/L) had no effect on corticosterone production.
出处
《河北医科大学学报》
CAS
1996年第5期257-258,共2页
Journal of Hebei Medical University
关键词
黄腐酸钠
颗粒细胞
肾上腺皮质
Sodium Fuliate
granulosa cell
adrenal cortical cell
estradiol
corticosterone
