摘要
牛血红细胞铜锌超氧物歧化酶（Ｃｕ·Ｚｎ－ＳＯＤ），经溶血，ＣｕＣｌ２处理及离子交换柱层析等步骤被纯化到高度均一程度．纯化酶在聚丙烯酰胺梯度凝胶图谱上的活性染色带与蛋白染色带相对应，在ＳＤＳ－ＰＡＧＥ图谱上呈现单一区带．元素分析表明该酶含有２个锌原子和多于２个的铜原子．分子量和亚基分子量分别为３３５００和１７８００．在紫外区酶有４个明显吸收峰，分别为２７４．６ｎｍ，２６６．２ｎｍ，２５８．８ｎｍ和２４５．４ｎｍ．该酶对热稳定，纯化后酶比活性为１４５２５ｕ／ｍｇ，活力回收率为４５％．本文对经ＣｕＣｌ２处理与氯仿－乙醇处理纯化的牛血Ｃｕ·Ｚｎ－ＳＯＤ进行比较，结果表明。
Bovine erythrocytes Cu·Zn-SOD was purified by hemolysating,treatment with CuCl 2 and DEAE-Cellulose ino exchange chromatography.On gel concentration gradient electrophoresis chromatogram,the stainde bands of the enzymic activity corresponded with that of enzymic protein.The enzyme contained 2 atons of Zn and more than 2 atoms of Cu.Its M.W.and its S.M.W.were 33500 and 17000 respectively.The enzyme exhibited fourth absorption peaks in U.V.at 274.6nm,266.2nm,258.8nm and 245.4nm,and was stalbe under the condition of 70℃.The purified enzyme was 14525 U/mg,the rote of recovery of activity was 45%. In this paper,we made a comparison between some properties of SOD from treatment with Alc-CHCl 3 and CuCl 2.The results show that the treatment of Alc-CHCl 3 influenced some properties of enzyme.
出处
《南京师大学报（自然科学版）》
CAS
CSCD
1996年第4期40-44,共5页
Journal of Nanjing Normal University(Natural Science Edition)
基金
江苏省教育委员会自然科学基金
关键词
牛
血红细胞
超氧物歧化酶
纯化
铜盐法
Copper/Zinc Superoxide Dismutase,Erythrocytes,CuCl 2,purity,property
